Activity and DNA contamination of commercial polymerase chain reaction reagents for the universal 16S rDNA real-time polymerase chain reaction detection of bacterial pathogens in blood

Diagnostic Microbiology and Infectious Disease
Helge MühlSamir G Sakka

Abstract

Universal 16S rRNA gene polymerase chain reaction (PCR) is a promising means of detecting bacteremia. Among other factors, the PCR reagents play a prominent role for obtaining a high sensitivity of detection. The reagents are ideally optimized with respect to the amplifying activity and absence of contaminating DNA. In this study, it was shown in a universal 16S rDNA real-time PCR assay that commercial PCR reagents can vary greatly among each other in these characters. Only 1 of the 5 reagents tested met the criteria of sensitive detection of pathogen DNA with a minimum of false-positive results. The reagent was validated by the detection of pathogens at low titers using bacterial DNA extracted from blood that was spiked with various Gram-positive and Gram-negative bacteria.

References

Jan 1, 1990·Journal of Clinical Microbiology·S E Phillips, J S Bradley
Aug 1, 1994·Journal of Clinical Microbiology·M S HughesR A Skuce
Apr 1, 1996·Journal of Clinical Microbiology·F B CogswellM T Philipp
Nov 14, 1997·Journal of Clinical Microbiology·D GoldenbergerM Altwegg
Jan 5, 2000·Journal of Clinical Microbiology·K Rantakokko-JalavaP Kotilainen
May 2, 2000·Journal of Clinical Microbiology·C E CorlessA J Fox
Jun 2, 2000·Journal of Clinical Microbiology·J A KelloggD A Bankert
Jul 6, 2000·Journal of Clinical Microbiology·J A Jordan, M B Durso
May 3, 2002·Clinical and Diagnostic Laboratory Immunology·Yoshimasa Yamamoto
Oct 17, 2002·FEMS Immunology and Medical Microbiology·E NavarroJ Solera
Apr 12, 2003·Journal of Clinical Microbiology·Alexandra HeiningerGerd Döring
May 5, 2005·FEMS Microbiology Letters·Masahiro GotoTadakatsu Yoneyama
Jun 1, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Robert A Wascher
Jul 8, 2005·Journal of Clinical Microbiology·Sven PoppertNele Wellinghausen
Aug 15, 2006·Journal of Microbiological Methods·Anne-Laure RoseyAgnès Ferroni
Nov 23, 2007·Journal of Clinical Microbiology·C A BalinskyL L Rodriguez
Dec 7, 2007·Journal of Microbiological Methods·Hans-Peter HorzGeorg Conrads

❮ Previous
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Citations

Jan 29, 2013·Applied Microbiology and Biotechnology·Franz SchnetzingerAndreas Nocker
Jan 13, 2010·Clinical Microbiology Reviews·Nicasio ManciniMassimo Clementi
Jun 19, 2013·PloS One·Alexander J ProbstChristine Moissl-Eichinger
Apr 11, 2014·Analytical and Bioanalytical Chemistry·Natalia SandetskayaDirk Kuhlmeier
May 23, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A J M LoonenA J C van den Brule
Apr 14, 2015·Expert Review of Molecular Diagnostics·Solomon MwaigwisyaJustin O'Grady
Mar 14, 2013·Expert Opinion on Medical Diagnostics·Antigone Kotsaki, Evangelos J Giamarellos-Bourboulis
Mar 15, 2011·Journal of Microbiological Methods·Herbert Wiesinger-MayrChrista Noehammer
Mar 15, 2011·Diagnostic Microbiology and Infectious Disease·Hector BonillaLouis D Saravolatz
Jul 10, 2014·Microbial Ecology·Adèle Mennerat, Ben C Sheldon
Jul 31, 2013·Transfusion·Pamela ClarkDamien Stark
Feb 22, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Christopher D SibleyDeirdre L Church
Dec 24, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Rémi Le GuernFrédéric Wallet
Dec 6, 2014·Journal of Human Evolution·Laura S WeyrichAlan Cooper
Nov 30, 2014·Biologicals : Journal of the International Association of Biological Standardization·Vyacheslav A FurtakVladimir Chizhikov
Aug 23, 2016·Gefässchirurgie : Zeitschrift für vaskuläre und endovaskuläre Chirurgie : Organ der Deutschen und der Österreichischen Gesellschaft für Gefässchirurgie unter Mitarbeit der Schweizerischen Gesellschaft für Gefässchirurgie·I TöpelM Steinbauer
Nov 23, 2006·Infectious Disease Clinics of North America·Raquel Cuchacovich
Jan 7, 2014·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·C L LiuZ Y Sun
Apr 22, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stefan DudliJeffrey C Lotz
Nov 2, 2016·PloS One·Nik VeldhoenCaren C Helbing
Jul 1, 2017·Journal of Clinical Laboratory Analysis·Yingping WuChen Cheng
Jul 10, 2018·Expert Review of Molecular Diagnostics·Jean Pierre RutangaStijn Deborggraeve
Mar 2, 2018·Clinical Microbiology Reviews·Mridu SinhaStephanie I Fraley
Nov 21, 2014·Journal of Clinical Microbiology·Chloé PlouzeauUNKNOWN Centre de Référence des Infections Ostéo-articulaires du Grand Ouest (CRIOGO) Study Team
Jan 30, 2015·Journal of Laboratory Automation·Katy ChunAngelika Niemz
May 17, 2014·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Guoquan ZhengYan Wang
Aug 4, 2019·BJOG : an International Journal of Obstetrics and Gynaecology·A A KupermanO Koren
Nov 10, 2017·Microbiology and Molecular Biology Reviews : MMBR·Christian MilaniMarco Ventura
Jun 20, 2020·Expert Review of Gastroenterology & Hepatology·Ian J O'NeillDouwe van Sinderen

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