Activity and functional properties of the isocitrate lyase in the cyanobacterium Cyanothece sp. PCC 7424

Microbiology
Marianne GründelRalf Steuer

Abstract

Cyanobacteria are ubiquitous photoautotrophs that assimilate atmospheric CO2 as their main source of carbon. Several cyanobacteria are known to be facultative heterotrophs that are able to grow on diverse carbon sources. For selected strains, assimilation of organic acids and mixotrophic growth on acetate has been reported for decades. However, evidence for the existence of a functional glyoxylate shunt in cyanobacteria has long been contradictory and unclear. Genes coding for isocitrate lyase (ICL) and malate synthase were recently identified in two strains of the genus Cyanothece, and the existence of the complete glyoxylate shunt was verified in a strain of Chlorogloeopsis fritschii. Here, we report that the gene PCC7424_4054 of the strain Cyanothece sp. PCC 7424 encodes an enzymatically active protein that catalyses the reaction of ICL, an enzyme that is specific for the glyoxylate shunt. We demonstrate that ICL activity is induced under alternating day/night cycles and acetate-supplemented cultures exhibit enhanced growth. In contrast, growth under constant light did not result in any detectable ICL activity or enhanced growth of acetate-supplemented cultures. Furthermore, our results indicate that, despite the presence of...Continue Reading

References

Jun 20, 1977·Archives of Microbiology·M J Ihlenfeldt, J Gibson
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jul 2, 1973·European Journal of Biochemistry·D Grierson, H Smith
Jan 1, 1972·Archiv für Mikrobiologie·J S Miller, M M Allen
Oct 1, 1967·Journal of Bacteriology·A J SmithR Y Stanier
Nov 1, 1967·Journal of General Microbiology·J Pearce, N G Carr
Sep 17, 1999·Bioinformatics·T A TatusovaJ A Ostell
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Jun 1, 2000·Nature·H OchmanE A Groisman
Nov 23, 2000·Current Opinion in Genetics & Development·J A Eisen
Feb 15, 2001·Trends in Plant Science·P J Eastmond, I A Graham
Apr 1, 1965·Journal of General Microbiology·P FAY
Jan 1, 1973·Plant Physiology·R F Troxler, J M Dokos
Apr 6, 2007·BMC Systems Biology·Steffen KlamtErnst D Gilles
Nov 3, 2009·Nucleic Acids Research·Mitsuteru NakaoYasukazu Nakamura
Dec 17, 2011·Science·Shuyi Zhang, Donald A Bryant
Nov 30, 2012·Nucleic Acids Research·Dennis A BensonEric W Sayers
Jan 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Patrick M ShihCheryl A Kerfeld
Jun 5, 2013·Indian Journal of Microbiology·Manojit DebnathSamit Ray
Apr 15, 2015·The Journal of Biological Chemistry·Shuyi Zhang, Donald A Bryant

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Citations

Mar 13, 2018·Critical Reviews in Microbiology·Alberto A Esteves-FerreiraRonan Sulpice
Sep 12, 2018·Annual Review of Microbiology·Stephen K Dolan, Martin Welch
Aug 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Nico J ClaassensArren Bar-Even

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