Activity-Based Concept to Screen Biological Matrices for Opiates and (Synthetic) Opioids

Clinical Chemistry
Annelies CannaertChristophe P Stove

Abstract

Detection of new highly potent synthetic opioids is challenging as new compounds enter the market. Here we present a novel screening method for the detection of opiates and (synthetic) opioids based on their activity. A cell-based system was set up in which activation of the μ-opioid receptor (MOR) led to recruitment of β-arrestin 2, resulting in functional complementation of a split NanoLuc luciferase and allowing readout via bioluminescence. Assay performance was evaluated on 107 postmortem blood samples. Blood (500 μL) was extracted via solid-phase extraction. Following evaporation and reconstitution in 100 μL of Opti-MEM® I, 20 μL was analyzed in the bioassay. In 8 samples containing synthetic opioids, in which no positive signal was obtained in the bioassay, quadrupole time-of-flight mass spectrometry revealed the MOR antagonist naloxone, which can prevent receptor activation. Hence, further evaluation did not include these samples. For U-47700 (74.5-547 ng/mL) and furanyl fentanyl (<1-38.8 ng/mL), detection was 100% (8/8) for U-47700 and 95% (21/22) for furanyl fentanyl. An analytical specificity of 93% (55/59) was obtained for the opioid negatives. From an additional 10 samples found to contain other opioids, 5 were corr...Continue Reading

References

Aug 9, 1996·The Journal of Biological Chemistry·D E KeithM von Zastrow
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J ZhangM G Caron
Oct 30, 2008·European Journal of Clinical Pharmacology·Janet K CollerAndrew A Somogyi
Dec 25, 2012·Bioorganic & Medicinal Chemistry Letters·Claire-Lise CianaChristoph Boss
Feb 7, 2016·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Anders HelanderOlof Beck
Jul 17, 2016·Forensic Science, Medicine, and Pathology·Xiulu RuanAlan D Kaye
Jul 20, 2016·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·K DomanskiK Tavakoli
Jul 28, 2016·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Aaron SchneirRobert L Fitzgerald
Aug 24, 2016·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Matthew J JonesSamuel J Stellpflug
Sep 4, 2016·Journal of Analytical Toxicology·Amanda L A MohrBarry K Logan
Oct 14, 2016·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Kathy T VoRaymond Y Ho
Jan 18, 2017·Journal of Analytical Toxicology·Davide GuerrieriRobert Kronstrand
Jan 26, 2017·Journal of Analytical Toxicology·Nicholas B Tiscione, Kristin Wegner
Apr 13, 2017·International Journal of Legal Medicine·Marek DziadoszJörg Teske
May 2, 2017·Journal of Analytical Toxicology·Barry K LoganMarilyn A Huestis
Jun 3, 2017·Journal of Analytical Toxicology·Dina M SwansonJulia M Pearson
Jun 8, 2017·Journal of Addiction Medicine·Matthew P PrekupecMichael H Baumann
Jul 18, 2017·Frontiers in Psychiatry·Jolanta B Zawilska
Sep 1, 2017·MMWR. Morbidity and Mortality Weekly Report·Raminta DaniulaityteRobert R Carlson
Oct 7, 2017·Journal of Analytical Toxicology·Kayla N EllefsenBrad J Hall
Oct 19, 2017·Journal of Analytical Toxicology·Timothy P RohrigTyson R Baird
Oct 19, 2017·Neuropharmacology·Patil ArmenianKara L Lynch
Mar 22, 2018·Clinical Chemistry·Annelies CannaertChristophe P Stove

❮ Previous
Next ❯

Citations

Mar 29, 2019·Critical Reviews in Toxicology·Annelies CannaertChristophe P Stove
Sep 5, 2019·British Journal of Clinical Pharmacology·Katharina Elisabeth GrafingerEvangelia Liakoni
Oct 3, 2019·Clinical Chemistry·Annelies CannaertChristophe P Stove
May 18, 2020·Drug Testing and Analysis·Sherif H HassanienJessica P Anand
Aug 1, 2020·Archives of Toxicology·Marthe M VandeputteChristophe P Stove
Sep 15, 2020·Analytical and Bioanalytical Chemistry·Lakshmi Vasudevan, Christophe P Stove
May 7, 2020·Analytical and Bioanalytical Chemistry·Michael F Santillo
Jul 16, 2019·Forensic Sciences Research·Inês TabarraEugenia Gallardo
Jul 14, 2020·Frontiers in Chemistry·Bronislav JurásekMartin Kuchař
Jun 20, 2019·International Journal of Molecular Sciences·Elise WoutersChristophe P Stove
Jun 6, 2018·Frontiers in Pharmacology·Annelies CannaertChristophe P Stove
Nov 28, 2020·Journal of Analytical Toxicology·Sarah M R Wille, Simon Elliott
Apr 7, 2021·Journal of Analytical Toxicology·Marthe M VandeputteChristophe P Stove
Sep 17, 2021·ACS Chemical Neuroscience·Karissa ResnikEmanuele Amorim Alves

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.