Activity-dependent heterogeneous populations of nitric oxide synthase neurons in the rat dorsal raphe nucleus

Brain Research
C O Okere, Barry D Waterhouse

Abstract

The brainstem dorsal raphe nucleus (DRN) contains an abundant distribution of nitric oxide (NO) synthase (NOS)-containing neuronal profiles in two distinct populations: faint- and intense-immunoreactive cells in midline (ventromedial and dorsomedial) and lateral wing subregions, respectively. This study tested the hypothesis that different functional dynamics underlie the topography of NOS-containing cells in the DRN rostrocaudal and mediolateral neuraxis by using a capsaicin challenge paradigm (50 mg/kg, subcutaneous). Compared with vehicle, capsaicin significantly and preferentially increased nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d, an index of constitutive NOS) reactivity in the rostral midline and caudal lateral wing subregions. Furthermore, capsaicin activated more Fos-positive cells than vehicle within all subregions of the DRN but with a caudal versus rostral predominance in activation pattern. In addition, a high proportion of capsaicin-induced Fos cells in the midline but almost none in lateral wing stained for NADPH-d. These observations suggest the existence of two functionally distinct populations of NOS neurons in the DRN. Furthermore, capsaicin increased galanin immunoreactivity with predom...Continue Reading

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Citations

Apr 8, 2014·Journal of Chemical Neuroanatomy·Rani K Vasudeva, Barry D Waterhouse
Dec 24, 2014·Pharmacology, Biochemistry, and Behavior·Anderson Manoel HerculanoCaio Maximino
Jun 7, 2011·Journal of Chemical Neuroanatomy·Rani K VasudevaBarry D Waterhouse
May 31, 2008·Neuroscience Letters·Bruna Cuccurazzu, Adam L Halberstadt
Apr 1, 2014·Progress in Neuro-psychopharmacology & Biological Psychiatry·Anderson Manoel Herculano, Caio Maximino
Jun 15, 2021·Journal of Chemical Neuroanatomy·Lucimário Thiago Félix de AraújoJudney Cley Cavalcante

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