Activity, expression and function of a second Drosophila protein kinase A catalytic subunit gene.

Genetics
A MeléndezD Kalderon

Abstract

The DC2 gene was isolated previously on the basis of sequence similarity to DC0, the major Drosophila protein kinase A (PKA) catalytic subunit gene. We show here that the 67-kD Drosophila DC2 protein behaves as a PKA catalytic subunit in vitro. DC2 is transcribed in mesodermal anlagen of early embryos. This expression depends on dorsal but on neither twist nor snail activity. DC2 transcriptional fusions mimic this embryonic expression and are also expressed in subsets of cells in the optic lamina, wing disc and leg discs of third instar larvae. A saturation screen of a small deficiency interval containing DC2 for recessive lethal mutations yielded no DC2 alleles. We therefore isolated new deficiencies to generate deficiency trans-heterozygotes that lacked DC2 activity. These animals were viable and fertile. The absence of DC2 did not affect the viability or phenotype of imaginal disc cells lacking DC0 activity or embryonic hatching of animals with reduced DC0 activity. Furthermore, transgenes expressing DC2 from a DC0 promoter did not efficiently rescue a variety of DC0 mutant phenotypes. These observations indicate that DC2 is not an essential gene and is unlikely to be functionally redundant with DC0, which has multiple uniqu...Continue Reading

Citations

Oct 31, 2006·Insect Molecular Biology·D EisenhardtG Leboulle
Jul 17, 2020·Journal of Cellular and Molecular Medicine·Hsun-Yu HuangHong-Lin Chan
Jul 17, 2001·Genetics·W J WolfgangM Forte
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·W J WolfgangM Forte
Dec 31, 1997·The Journal of Biological Chemistry·C R GuthrieG S McKnight
Jun 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xiaohong LiChristopher R Burrow
May 10, 2002·Genes & Development·Offer Gerlitz, Konrad Basler
Mar 12, 2011·Biochimica Et Biophysica Acta·Xiaohong Li

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