Activity of a sodium-dependent vitamin C transporter (SVCT) in MDCK-MDR1 cells and mechanism of ascorbate uptake.

International Journal of Pharmaceutics
Shuanghui LuoAshim K Mitra

Abstract

The objective of this research was to functionally characterize sodium-dependent vitamin C transporter (SVCT) in MDCK-MDR1 cells and to study the effect of substituted benzene derivatives on the intracellular accumulation of ascorbic acid (AA). Mechanism of AA uptake and transport was delineated. Uptake of [(14)C]ascorbic acid ([(14)C]AA) was studied in the absence and presence of excess unlabelled AA, anion transporter inhibitors, and a series of mono- and di-substituted benzenes. Transepithelial transport of [(14)C]AA across polarized cell membrane has been studied for the first time. Role of cellular protein kinase-mediated pathways on the regulation of AA uptake has been investigated. The cellular localizations of SVCTs were observed using confocal microscopy. Uptake of AA was found to be saturable with a K(m) of 83.2muM and V(max) of 94.2pmol/min/mg protein for SVCT1. The process was pH, sodium, temperature, and energy-dependent. It was under the regulation of cellular protein kinase C (PKC) and Ca(2+)/CaM mediated pathways. [(14)C]AA uptake was significantly inhibited in the presence of excess unlabelled AA and a series of electron-withdrawing group, i.e., halogen- and nitro-substituted benzene derivatives. AA appears to ...Continue Reading

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Citations

Feb 2, 2012·Chemical & Pharmaceutical Bulletin·Jintaek KimMinoru Maeda
Mar 20, 2013·Molecular Aspects of Medicine·Marc BürzleMatthias A Hediger
Mar 20, 2013·Molecular Aspects of Medicine·Matthias A HedigerElspeth A Bruford
Jun 14, 2012·International Journal of Pharmaceutics·Aswani Dutt VadlapudiAshim K Mitra
Nov 26, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zheng-Gen LiaoXin-Li Liang
Jul 13, 2017·Antioxidants & Redox Signaling·Orazio CantoniMara Fiorani
Aug 2, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaocong PangGuan-Hua Du
Jul 13, 2018·Frontiers in Molecular Biosciences·Vito ScaleraAnnamaria Tonazzi
Jun 3, 2020·Topics in Companion Animal Medicine·Daniel S GordonKarina J Creighton
Jan 24, 2021·International Journal of Biological Macromolecules·Zhuo HanZekun Guo

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