Activity of carbonyl cyanide-3-chlorophenylhydrazone on biofilm formation and antimicrobial resistance inPseudomonas aeruginosausing quantum dots-meropenem conjugates as nanotools.

Methods and Applications in Fluorescence
Valdemir V Silva JúniorMaria A V Maciel

Abstract

Hospital infections associated with multidrug-resistant (MDR)Pseudomonas aeruginosaare a worldwide public health problem. Efflux systems and biofilm formation are mechanisms related to resistance to carbapenemics. In this study, quantum dots (QDs) were used to evaluate the effect of carbonyl cyanide-3-chlorophenylhydrazone (CCCP), an efflux pump system inhibitor, on biofilm formation and antimicrobial resistance profile ofP. aeruginosastrains. For this, QDs were covalently conjugated to meropenem (MPM) and incubated with aP. aeruginosaresistant isolate (P118) or a control sensitive strain (ATCC Pa27853). P118 was also analyzed with conjugates after previous CCCP efflux inhibitor incubation. Fluorescence microscopy images showed that both sensitive and resistant bacteria were efficiently labeled. Nevertheless, P118 isolates presented fluorescent cell agglomerates, suggesting biofilm formation. The addition of the CCCP changed the labeling profile of the resistant isolate, and the absence of agglomerates was observed, indicating no biofilm formation. Genetic assays revealed the presence ofMexAandMexEgenes encoding channel proteins from efflux pump systems in both resistant and sensitive strains. Disk-diffusion and broth microdilu...Continue Reading

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