Abstract
The signal molecule, 3-oxo-C12-homoserine lactone (3-oxo-C12-HSL), is similar to a mammalian hormone in bacteria. Although most studies have examined the effects of high 3-oxo-C12-HSL concentrations (>200 μM) on mammalian cellular functions because ~600 μM 3-oxo-C12-HSL can be secreted in biofilms of Pseudomonas aeruginosa grown in vitro, we previously showed that a low 3-oxo-C12-HSL concentration (30 μM) induces the apoptosis of undifferentiated Caco-2 cells through suppressing Akt activity. Here, we found that a low concentration of 3-oxo-C12-HSL-activated ERK1/2 in undifferentiated Caco-2 cells. Incubating cells with the ERK pathway inhibitor U0126 for 30 min alleviated the mucin 3 (MUC3) expression suppressed by 3-oxo-C12-HSL, and the upregulation of MUC3 expression induced by a 48-h incubation with U0126-reduced cell death. Thus, altered MUC3 expression caused by long-term attenuated ERK1/2 activity might correlate with the death of undifferentiated Caco-2 cells induced by 3-oxo-C12-HSL.
References
May 1, 1998·Annals of Surgery·A PierroD A Lloyd
Apr 13, 1999·The American Journal of Physiology·D R MackM A Hollingsworth
Nov 27, 1999·Science·C RommelD J Glass
Nov 27, 1999·Science·S Zimmermann, K Moelling
Mar 10, 2001·Environmental Microbiology·T S CharltonS Kjelleberg
Aug 22, 2001·Journal of Bacteriology·T KöhlerJ C Pechere
Dec 1, 2001·Gastroenterology·E H RingsP G Traber
Mar 16, 2002·Infection and Immunity·David L EricksonDouglas G Storey
May 13, 2003·Gut·D R MackM A Hollingsworth
Jun 24, 2003·Biochimica Et Biophysica Acta·Laurie L Shekels, Samuel B Ho
Sep 16, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·C Caballero-FrancoK Chadee
Nov 15, 2006·Gastroenterology·Samuel B HoLaurie L Shekels
Dec 13, 2006·FEBS Letters·Elena VikströmKarl-Eric Magnusson
Nov 29, 2008·Experimental Cell Research·Elena VikströmKarl-Eric Magnusson
Oct 17, 2012·PLoS Pathogens·Thommie KarlssonElena Vikström
Jan 15, 2013·The Journal of Biological Chemistry·Qiong PanRongquan Wang
Apr 20, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sung Yong EumMichal Toborek
May 23, 2014·American Journal of Physiology. Cell Physiology·Ryoko TaguchiHitoshi Miyazaki
Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Ga-Hyun JoeSatoshi Ishizuka