Activity of esterases from different tissues of freshwater fish and responses of their isoenzymes to inhibitors

Journal of Toxicology and Environmental Health
S N Li, D F Fan

Abstract

Activity of nonspecific esterase from different tissues (i.e., liver, gallbladder, heart, intestine, and muscle) of five species of freshwater fish, namely, topmouth gudgeon (Pseudorasbora parva), goldfish (Carassius auratus), nile tilapia (Tilapia nilotica), mosquitofish (Gambusia affinis), and rainbow trout (Salmo gairdneri) was tested using alpha-naphthyl acetate as substrate. The results indicated that activity of the enzyme was mainly concentrated in the digestive system (i.e., intestine, liver, bile). The overall activity was highest in nile tilapia, followed by mosquitofish, topmouth gudgeon, goldfish, and lowest in rainbow trout. Electrophoresis and the following in vitro treatment of the isoenzymes with triphenol phosphate (TPP, an inhibitor of carboxylesterase) indicated the TPP-sensitive esterase was mainly distributed in liver of the five species. The enzyme was not found in the other five tissues (including gill) except in gallbladder of topmouth gudgeon and goldfish. The correlation was obviously improved between susceptibility and detoxification capacity if activity of the TPP-sensitive esterase was employed instead of that of the nonspecific esterase to make the comparison. In vitro treatment of nonspecific este...Continue Reading

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Citations

Sep 14, 2010·Environmental Toxicology and Chemistry·Craig M ButtScott A Mabury
Nov 28, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Lígia Maria MolinariBenedito Prado Dias Filho
Sep 3, 2020·EFSA Journal·UNKNOWN EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP)Matteo Lorenzo Innocenti
May 14, 2018·Fish Physiology and Biochemistry·Patrick G PumputisNiels C Bols
Feb 1, 2017·EFSA Journal·UNKNOWN EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP)Andrew Chesson
Sep 14, 2018·Chemosphere·Carlos Eduardo Delfino Vieira, Claudia Bueno Dos Reis Martinez

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