Activity of Euglena gracilis chloroplast ribosomes with procaryotic and eucaryotic initiation factors

Archives of Biochemistry and Biophysics
M C Graves, Linda L Spremulli

Abstract

A method that permits the preparation of Euglena gracilis chloroplast 30 S ribosomal subunits that are largely free of endogenous initiation factors and that are active in the binding of fMet-tRNA in response to poly(A, U, G), has been developed. These 30 S subunits have been tested for activity in initiation complex formation with initiation factors from both procaryotes and eucaryotes. We have observed that Escherichia coli IF-2 binds fMet-tRNA nearly as well to Euglena chloroplast ribosomal subunits as it does to its homologous subunits. Neither wheat germ eIF-2 nor Euglena eIF-2A can bind fMet-tRNA efficiently to Euglena chloroplast or E. coli 30 S subunits although both are active with wheat germ 40 S ribosomal subunits. Euglena chloroplast 68 S ribosomes will also bind the initiator tRNA. Both E. coli IF-2 and E. coli IF-3 stimulate this reaction on chloroplast ribosomes with approximately the same efficiency as they do on their homologous ribosomes. E. coli IF-1 enhances the binding of fMet-tRNA to the chloroplast 68 S ribosomes when either IF-2 or IF-3 is limiting. The chloroplast ribosomes unlike E. coli ribosomes show considerable activity over a broad range of Mg2+ ion concentrations.

References

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Citations

Feb 26, 2009·Biochemistry·Brooke E Christian, Linda L Spremulli
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Aymen S YassinRajendra K Agrawal
Jul 9, 2010·The Journal of Biological Chemistry·Brooke E Christian, Linda L Spremulli
Oct 13, 2004·Nucleic Acids Research·Angela C Spencer, Linda L Spremulli
Dec 8, 2009·Biochemical and Biophysical Research Communications·Brooke E ChristianLinda L Spremulli
Nov 15, 1984·Archives of Biochemistry and Biophysics·A Pramanik, I Schwartz
Jun 7, 2005·Biochimica Et Biophysica Acta·Angela C Spencer, Linda L Spremulli
Oct 22, 2008·Journal of Molecular Biology·Md Emdadul Haque, Linda L Spremulli

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