Activity of human immunodeficiency virus type 1 promoter/TAR regions and tat1 genes derived from individuals with different rates of disease progression

Virology
Frank KirchhoffR C Desrosiers

Abstract

Different rates of disease progression may be associated with different human immunodeficiency virus type 1 (HIV-1) promoter and/or transactivator activities. We therefore analyzed the sequences and activities of the first exon of Tat, tat1, and the promoter/trans-acting responsive (TAR) regions amplified directly from peripheral blood mononuclear cells obtained from five long-term nonprogressors and eight progressing HIV-1-infected individuals. The majority of tat1 alleles and promoter/TAR regions from all patients were intact and showed comparable activities in transient reporter assays. A substantial number of point mutations and some length variations were observed in the promoter/TAR region. In a single nonprogressor, the Sp1 binding site 3 was consistently altered and the transcriptional activity in the presence of Tat was diminished. Some LTR clones from a rapid progressor contained a fourth Sp1 binding site, which was associated with an elevated basal promoter activity. These data suggest that defects in the promoter/TAR region or tat1 are rare and that different promoter/transactivator activities are not commonly associated with different progression rates.

References

Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C DingwallR Valerio
Jan 26, 1995·The New England Journal of Medicine·G PantaleoL K Schrager
Jan 26, 1995·The New England Journal of Medicine·F KirchhoffR C Desrosiers
Dec 1, 1993·AIDS Research and Human Retroviruses·E SabinoA Mayer

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Citations

Jul 6, 2013·AIDS Research and Human Retroviruses·Jessica M MatesJesse J Kwiek
Feb 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lindsey ReynoldsYen Choo
Oct 26, 2010·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yujie LiuBrian Wigdahl
Jun 16, 2010·Bio Systems·Susanna L LamersMichael S McGrath
Oct 15, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Ivan Sadowski, David A Mitchell
Dec 17, 2003·Virology·Kirsten Hiebenthal-MillowFrank Kirchhoff
Sep 10, 2003·Journal of Leukocyte Biology·Olivier RohrEvelyne Schaeffer
Mar 5, 2020·Reviews in Medical Virology·Jason T BlackardBenjamin L Laskin
Jan 24, 1998·Science·C F Farthing, J L Sullivan

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