Activity of inducible and neuronal nitric oxide synthases in colonic mucosa predicts progression of ulcerative colitis

The American Journal of Gastroenterology
Luis MenchénPedro Lorenzo

Abstract

The present study analyzes inducible and neuronal nitric oxide synthase activity and expression in colonic mucosa of patients with ulcerative colitis, and correlates them with the progression of disease extent. Thirty patients with ulcerative colitis were included. Synthases activity and expression were analyzed both in inflamed and noninflamed mucosa. After 2 yr, disease extent was determined and compared with extent at inclusion. Ca(2+)-independent activity, expressed as median with (interquartile range), in inflamed mucosa was higher than in noninflamed and control mucosa (102 (165-66), 24 (50-3), 1 (2.5-0.1) pmol.min(-1) mg prot(-1), respectively, p < 0.005), whereas Ca(2+)-dependent activity was significantly lower in inflamed than in noninflamed and control mucosa. Western blot analysis identified inducible and neuronal isoforms and confirmed these differences. Patients with more extended disease after 2 yr had higher levels of Ca(2+)-independent activity in noninflamed mucosa at inclusion and lower levels of Ca(2+)-dependent activity than patients with persistence of similar extent of inflammation (50 (78-29) vs 8 (30-0.1), p < 0.005; 51 (100-36) vs 150 (156-106), p < 0.05, respectively). Values of Ca(2+)-independent act...Continue Reading

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Citations

Oct 19, 2010·World Journal of Gastroenterology : WJG·Lucía MárquezJuan C Leza
Apr 2, 2011·World Journal of Gastroenterology : WJG·Carla CirilloRosario Cuomo
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Apr 8, 2011·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M BagyánszkiA B A Kroese
Nov 2, 2011·Scandinavian Journal of Gastroenterology·Jon Florholmen, Walter Fries
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Mar 27, 2021·Cellular and Molecular Life Sciences : CMLS·Amy Marie HollandWerend Boesmans

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