PMID: 44600Jan 1, 1979

Activity of L-asparaginase in cells of Streptomyces karnatakensis

Zentralblatt Für Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. Zweite Naturwissenschaftliche Abteilung: Mikrobiologie Der Landwirtschaft Der Technologie Und Des Umweltschutzes
S A Mostafa

Abstract

Eight isolates capable of producing varying quantities of L-asparaginase and all identified as members of the genus Streptomyces were isolated from the soil and a suitable technique for the assay of intracellular L-asparaginase in actinomycetes was developed. The most potent L-asparaginase producer was identified as a strain of Streptomyces karnatakensis. Static cultures of S. karnatakensis showed maximum enzyme activity with almost maximum growth while shaken cultures exhibited their activity after 48 hours of growth. This phenomenon is discussed in terms of possible feedback mechanism and/or the biosynthesis of certain pigments. L-asparaginase of S. karnatakensis proved to be mostly intracellular and the presence of L-asparagine in the culture medium though, stimulating yet not essential for the enyzme biosynthesis. Cells grown on L-asparagine showed amidase activity with other amides but at a reduced rate.

References

May 24, 2012·TheScientificWorldJournal·Alapati Kavitha, Muvva Vijayalakshmi
Mar 13, 2014·TheScientificWorldJournal·Neelima DeshpandeManasi Agashe
Nov 9, 2015·Applied Biochemistry and Biotechnology·Tahira BatoolMashitah M Yusoff

Related Concepts

Asparagine
Amides
Protoplasm
Pentosuria
Asparaginase
Amide local anesthetics
Enzyme Activity
Asparaginase medac
Blood Enzyme Activity (Lab Test)
Actinobacteria class

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