Dec 9, 1997

Activity of mitochondrial respiratory chain enzymes after transient focal ischemia in the rat

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
L CanevariB K Siesjö

Abstract

Previous results demonstrated that after 2-hour middle cerebral artery occlusion (MCAO) in the rat, 1- to 2-hour recirculation temporarily restored the bioenergetic state and mitochondrial function, but secondary deterioration took place after 4 hours. The authors measured the activity of mitochondrial respiratory chain complexes, citrate synthase, and glutamate dehydrogenase as possible targets of secondary damage. Focal and penumbral tissues were sampled in the control condition, after 2 hours of MCAO, and after 1, 2, or 4 hours of postischemic recirculation; two groups were treated with alpha-phenyl-N-tert-butyl-nitrone (PBN). Complex IV activity transiently decreased after MCAO, but after recirculation all measured activities returned to control values.

  • References23
  • Citations27

References

  • References23
  • Citations27

Citations

Mentioned in this Paper

Ischemia
Cytochrome c Oxidase Subunit VIa
Cyclic N-Oxides
phenyl-N-tert-butylnitrone, (Z)-isomer
Nitrogen Oxides
Mitochondria
Crescendo Transient Ischemic Attacks
Oxygen Consumption
Rats, Wistar
Cs

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