PMID: 6111352Jan 26, 1981Paper

Activity of N-acetylmuramoyl-L-alanine amidase in phospholipidic environments

Biochimica Et Biophysica Acta
E VanderwinkelL de Tanhoffer de Volcsey

Abstract

A purified preparation of N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), a murein hydrolase from Escherichia coli, was found to lose its activity during incubation in the presence of bacterial phospholipid suspensions. Whether it was co-dispersed with the phospholipids or added to sonicated phospholipid suspension, the enzyme was inhibited (or inactivated) from the first minutes of incubation at 37 degree C. As phosphatidylglycerol/cardiolipin ratio of the phospholipid suspension as increased (all other things being equal), a further decrease of amidase activity was observed. The highest losses of activity were found after co-dispersion of the enzyme and the substrate together with the phospholipids, the resulting suspension being formed of larger multilayered vesicles, as revealed by electron microscopy. In these conditions, the effect on enzyme activity was only partially accounted for by the proportion of the enzyme that was entrapped in the vesicles. The entrapment capacity of the enzyme (using a 35S-labelled enzyme preparation) and of the substrate (3H-labelled) by the multilamellar phospholipidic vesicles did not significantly change as a function of their relative content of phosphatidylglycerol and cardiolipin. The p...Continue Reading

References

Jan 14, 1975·Biochimica Et Biophysica Acta·M ImaiS Nojima
Jan 15, 1979·The Biochemical Journal·K A WebsterD Papahadjopoulos
Apr 1, 1976·Journal of Bacteriology·R F ClevelandG D Shockman
Sep 1, 1976·Journal of Bacteriology·R F ClevelandG D Shockman
Jan 1, 1976·Methods in Enzymology·G Gregoriadis
Oct 15, 1975·European Journal of Biochemistry·J van HeijenoortY van Heijenoort
Sep 16, 1974·European Journal of Biochemistry·E W GoodellR M Teather
Sep 9, 1967·Biochimica Et Biophysica Acta·D Papahadjopoulos, N Miller
May 10, 1974·Annals of the New York Academy of Sciences·G D ShockmanM L Higgins
Sep 9, 1967·Biochimica Et Biophysica Acta·D Papahadjopoulos, J C Watkins

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Citations

Dec 1, 1984·Archives internationales de physiologie et de biochimie·R A PizarroL V Orce
Dec 1, 1988·Archives internationales de physiologie et de biochimie·R A PizarroL V Orce
Jul 6, 1990·Biochimica Et Biophysica Acta·E VanderwinkelJ P ten Have

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