Activity of squalene-hopene cyclases in bicontinuous microemulsions

Colloids and Surfaces. B, Biointerfaces
Anne K SteudleCosima Stubenrauch

Abstract

The paper at hand deals with biocatalysis in bicontinuous microemulsions. The latter consist of a dynamic network of oil and water domains separated by a monolayer of surfactant molecules, i.e. the interfacial layer. A microemulsion with the composition buffer--n-octane--nonionic surfactant was tested as reaction medium for an enzyme-catalysed reaction with a focus on the conversion of hydrophobic substrates, which are difficult to convert in aqueous buffer solutions. For the study at hand, we chose to investigate the activity of the squalene-hopene cyclase from Alicyclobacillus acidocaldarius (AacSHC) towards its natural substrate squalene in bicontinuous microemulsions. Firstly, the study revealed that the activity of AacSHC depends linearly on the enzyme concentration. Secondly, a hyperbolic curve was found for the dependence of the activity on the substrate concentration and a saturation of the AacSHC at substrate concentrations above 20mM was observed. Thirdly, the composition of the interfacial layer was found to have no significant influence on the activity or on the conformation of AacSHC. Surprisingly and unexpectedly, a distinctly enhanced selectivity towards hopene was discovered in the microemulsion. To conclude, bi...Continue Reading

References

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Feb 26, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Mireia SubinyaSandra Engelskirchen
Dec 17, 2014·Chemistry : a European Journal·Anne K SteudleCosima Stubenrauch
May 15, 2015·Colloids and Surfaces. B, Biointerfaces·Mireia SubinyaCosima Stubenrauch

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Citations

Jun 13, 2018·Soft Matter·Douglas G HayesRan Ye
Feb 2, 2021·Journal of Colloid and Interface Science·V K SharmaE Mamontov

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