PMID: 11607226Oct 15, 1991Paper

Activity of the Agrobacterium T-DNA transfer machinery is affected by virB gene products

Proceedings of the National Academy of Sciences of the United States of America
J E WardA N Binns

Abstract

The oriT (origin of transfer) sequence and mob (mobilization) genes of plasmid RSF1010 can functionally replace transfer DNA (T-DNA) borders to generate an RSF1010 intermediate transferable to plants through activities of the tumor-inducing (Ti)-plasmid virulence (vir) genes of Agrobacterium tumefaciens. Because the Ti plasmid virB gene products are hypothesized to form a membrane-localized T-DNA transport apparatus, we investigated whether specific virB genes were needed for RSF1010 transfer. Here we report that transformation of Nicotiana tabacum leaf explants by an RSF1010-derivative plasmid (pJW323) requires the essential virulence genes virB9, virB10, and virB11, consistent with the hypothesis that both the T-DNA and RSF1010 transfer intermediates utilize the same transport machinery. Further, while pJW323 is transferred into plant cells by Agrobacterium strains harboring both pJW323 and pTiA6, the initiation of crown gall tumors (i.e., T-DNA transfer) is greatly suppressed. Coordinate overexpression of the virB9, virB10, and virB11 gene products relieves pJW323-mediated oncogenic suppression and restores tumorigenicity, but does not increase the transfer frequency of pJW323 into plant cells. We propose that the virB9, vir...Continue Reading

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Citations

Jul 1, 1996·World Journal of Microbiology & Biotechnology·K Weising, G Kahl
Dec 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xiaozhen HuAndrew N Binns
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J BohneA N Binns
Aug 21, 2015·Research in Microbiology·Katsunori SuzukiShinji Yamamoto
Jan 28, 2014·Biochemistry. Biokhimii︠a︡·M I Chumakov
Nov 3, 2007·Microbiology·Khaled A Aly, Christian Baron
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Cristina E Alvarez-Martinez, Peter J Christie
Sep 22, 2001·Journal of Bacteriology·E SagulenkoP J Christie
Mar 1, 1996·Journal of Bacteriology·K J Fullner, E W Nester
Jan 13, 2018·Microbiology Spectrum·María Getino, Fernando de la Cruz
May 29, 1992·Science·A BeijersbergenP J Hooykaas

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