Nov 1, 1981

Activity of the enzyme of the 2d step of flavinogenesis, 2,5-diamino-6-hydroxy-4-ribosylaminopyrimidine-5'-phosphate reductase, in Pichia guilliermondii yeasts

Mikrobiologiia
G M ShavlovskiĭA E Zakal'skiĭ

Abstract

The activity of 2,5-diamino-6-hydroxy-4-ribosylaminopyrimidine-5'-phosphate reductase, an enzyme of the second step of flavinogenesis, was studied in the yeast Pichia guilliermondii using a new fluorimetric technique. The activity of the enzyme was found in extracts of the wild-type strain and the genotype rib mutants but not in extracts of the FM 1-4 (rib 2) mutant. Interallelic complementation was revealed in the gene rib 2. The activity of the enzyme was identical in extracts of the cells grown in the medium with either a high or a low iron content which synthesized riboflavin at a low or a high rate, respectively. Therefore, the synthesis of this enzyme in P. guilliermondii is not regulated with iron.

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Mentioned in this Paper

Pichia guilliermondii
Yeasts
Riboflavin
Rib 2
Sugar Alcohol Dehydrogenases
Mutant
RIB2
Cochliobolus
Flavins
Riboflavin Assay

About this Paper

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