Activity of Thymus capitellatus volatile extract, 1,8-cineole and borneol against Leishmania species

Veterinary Parasitology
M MachadoM C Sousa

Abstract

In the search for new leishmanicidal agents, Thymus capitellatus Hoffmanns. & Link (family Lamiaceae) volatile extract and its major compounds, 1,8-cineole and borneol, were tested against Leishmania infantum, Leishmania tropica and Leishmania major. Plant volatile extract (essential oil) was analysed by GC and GC-MS and the activity of essential oil on Leishmania promastigotes viability was assessed using tetrazolium-dye colorimetric method (MTT). The MTT test was also used to assess the cytotoxicity of essential oil on macrophages and bovine aortic endothelial cells. Effects on parasites were also analyzed by flow cytometry in order to assess mitochondrial transmembrane electrochemical gradient (JC-1), analyze phosphatidylserine externalization (annexin V-FITC, propidium iodide) and evaluate cell cycle (DNase-free, RNase, PI). Morphological and ultrastructural studies were performed by light, scanning and transmission electron microscopy. T. capitellatus volatile extract exhibited anti-parasite activity on Leishmania species, with IC50 values ranging from 35 to 62 μg/ml. However, major compounds 1,8-cineole and borneol did not showed biological activity suggesting that these monoterpenes are not responsible for the antileishm...Continue Reading

References

Mar 10, 2001·Clinical and Diagnostic Laboratory Immunology·M C SousaJ Poiares-Da-Silva
May 4, 2001·Micron : the International Research and Review Journal for Microscopy·J Dobrucki, Z Darzynkiewicz
Jun 25, 2002·Veterinary Parasitology·Gad Baneth, Susan E Shaw
Sep 19, 2003·International Journal of Antimicrobial Agents·Clara Leandro, Lenea Campino
Jul 27, 2004·Antimicrobial Agents and Chemotherapy·Silvia Orenes LorenteIan H Gilbert
Aug 16, 2005·Trends in Parasitology·Jean-Paul AnthonyHuw Smith
Aug 17, 2005·Veterinary Dermatology·Chiara Noli, Silvia T Auxilia
Dec 14, 2005·Parasitology International·Tânia Ueda-NakamuraCelso Vataru Nakamura
Jan 19, 2006·Clinical Microbiology Reviews·Simon L CroftAlan H Fairlamb
Dec 28, 2006·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·V LakshmiA Dube
Mar 14, 2007·International Journal of Antimicrobial Agents·Sonimar NateraAlicia Ponte-Sucre
Mar 16, 2007·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·H NakayamaP M Loiseau
Nov 13, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F BakkaliM Idaomar
Dec 22, 2007·Journal of Agricultural and Food Chemistry·Valtcho D ZheljazkovShabana I Khan
Jun 3, 2008·Trends in Parasitology·Gad BanethLluis Ferrer
Jul 8, 2008·Trends in Parasitology·Guadalupe MiróGad Baneth
Jan 22, 2010·The Journal of Antimicrobial Chemotherapy·Karin SeifertSimon L Croft
Apr 21, 2010·International Journal of Antimicrobial Agents·Rupashree SenMitali Chatterjee
Apr 30, 2010·Memórias do Instituto Oswaldo Cruz·Lianet MonzoteMigdalia Miranda
Apr 30, 2010·Memórias do Instituto Oswaldo Cruz·Patricia EscobarElena Stashenko
Aug 24, 2010·International Journal of Antimicrobial Agents·Jose A Ruiz Postigo
Sep 25, 2010·Annals of Tropical Medicine and Parasitology·A O SantosC V Nakamura
Sep 28, 2010·Veterinary Parasitology·C MaiaL Campino
Feb 15, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dominique F Moura do CarmoJefferson Rocha de Andrade Silva
May 12, 2012·Veterinary Parasitology·Sofia CortesLenea Campino
Oct 17, 2012·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Fernanda Cristina Macedo RondonHilton César Rodrigues Magalhães
Apr 24, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Niko S RadulovićVidosava B Djordjević

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Citations

Jan 4, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Javad Sharifi-RadMarcello Iriti
Jun 28, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thanh Binh LeJoëlle Quetin-Leclercq
Jun 26, 2018·Andrologia·Rosana CavalleriIvan Cunha Bustamante-Filho
Apr 28, 2020·Pathogens and Global Health·Sofia CortesLenea Campino
Oct 6, 2016·Phytotherapy Research : PTR·Nazif UllahMuhammad Hamayun
Nov 19, 2014·Medicines·Lianet MonzoteWilliam N Setzer
May 17, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Audrey R S T SilvaSilvio S Dolabella
May 28, 2019·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Daiane Barros Dias MendonçaJose Angelo Lauletta Lindoso
Dec 1, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Renata Mondêgo-OliveiraAna Lucia Abreu-Silva
Nov 5, 2020·Anais Da Academia Brasileira De Ciências·Katily L G PereiraRicardo Scher
May 23, 2020·Journal of Ethnopharmacology·Raissa Couto SantanaLucia H Pinto-da-Silva

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