Activity, peroxide compound formation, and heme d synthesis in Escherichia coli HPII catalase

Archives of Biochemistry and Biophysics
Christian ObingerP C Loewen

Abstract

Wild-type Escherichia coli HPII catalase (heme d containing) has 15% the activity of beef liver enzyme per heme. The rate constant for compound I formation with H2O2 is 1.3 x 10(6) M(-1) s(-1). HPII compound I reacts with H2O2 to form O2 with a rate constant of 1.8 x 10(6) M(-1) s(-1). Forty percent of HPII hemes are in the compound I state during turnover. Compound I is reduced by ethanol and formate at rates of 5 and 13 M(-1) s(-1) (pH 7.0), respectively. Incubation of HPII compound I with ferrocyanide and ascorbate does not form a compound II species. Mutation of His128 to alanine or asparagine gives inactive protoheme proteins. Mutation of Asn201 gives partially active heme d forms. Asn201Ala has 24%, Asn201Asp 10%, and Asn201Gln 0.4% of wild-type activity. Asn201His contains protoheme when isolated and converts this via protoheme compound I to a heme d species. Both distal heme cavity residues His128 and Asn201 are implicated in catalytic activity, compound I formation, and in situ heme d biosynthesis. HPII Asn201, like the corresponding residue in protoheme catalases, may promote H+ transfer to His128 imidazole, facilitating (i) peroxide anion binding to heme and (ii) stabilization of a transition state for heterolytic cl...Continue Reading

References

Jul 1, 1979·Physiological Reviews·B ChanceA Boveris
Jan 1, 1992·The Biochemical Journal·M J Davies, A Puppo
Jan 1, 1992·Annual Review of Pharmacology and Toxicology·P R Ortiz de Montellano
Jul 1, 1986·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·P C Loewen, J Switala
Sep 5, 1985·Journal of Molecular Biology·I Fita, M G Rossmann
Jul 1, 1970·Physiological Reviews·A Deisseroth, A L Dounce
Mar 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D DolphinR H Felton
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H N Kirkman, G F Gaetani
Oct 25, 1981·Journal of Molecular Biology·M R MurthyM G Rossmann
May 15, 1995·Structure·J BravoI Fita
Dec 5, 1996·Biochimica Et Biophysica Acta·M MajP C Loewen
Aug 1, 1955·Archives of Biochemistry and Biophysics·Y OGURA
Mar 9, 1964·Biochimica Et Biophysica Acta·P NICHOLLS
Jun 1, 1955·The Biochemical Journal·D KEILIN, E F HARTREE
Jul 2, 1962·Biochimica Et Biophysica Acta·P NICHOLLS
Apr 1, 1950·The Biochemical Journal·B CHANCE

❮ Previous
Next ❯

Citations

Nov 7, 2015·PLoS Computational Biology·Kristin J Adolfsen, Mark P Brynildsen
Feb 14, 2020·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Fatemeh NazariElham Mohajel Kazemi
Jun 5, 2003·The Journal of Biological Chemistry·Pedro EchaveJoaquim Ros
Mar 23, 2013·Acta Crystallographica. Section D, Biological Crystallography·Yonca YuzugulluMichael J McPherson
Jul 25, 2019·Environmental Science and Pollution Research International·Samaneh Torbati
Jul 7, 2019·Research in Microbiology·Robert K PooleMark Shepherd
Apr 4, 2019·Archives of Biochemistry and Biophysics·Andrés Zárate-RomeroEnrique Rudiño-Piñera
Aug 26, 2020·Analytical Biochemistry·Michael ChabotRakesh Mogul
Feb 5, 1999·Journal of Molecular Biology·M J MatéI Fita
Aug 14, 1999·Progress in Biophysics and Molecular Biology·M Zámocký, F Koller
Apr 1, 2007·EcoSal Plus·Samuel I Beale
Mar 9, 2011·Journal of the American Chemical Society·M Alfonso-PrietoJ Blumberger
Sep 27, 2001·Journal of the American Chemical Society·S G KalkoM Orozco
Apr 13, 2005·Biochemistry·Prashen ChelikaniPeter C Loewen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.