PMID: 3457004Apr 1, 1986

Activity, reconstitution, and accumulation of nitrogenase components in Azotobacter vinelandii mutant strains containing defined deletions within the nitrogenase structural gene cluster

Journal of Bacteriology
A C RobinsonD R Dean


The Azotobacter vinelandii genes encoding the nitrogenase structural components are clustered and ordered: nifH (Fe protein)-nifD (MoFe protein alpha subunit)-nifK (MoFe protein beta subunit). In this study various A. vinelandii mutant strains which contain defined deletions within the nitrogenase structural genes were isolated and studied. Mutants deleted for the nifD or nifK genes were still able to accumulate significant amounts of the unaltered MoFe protein subunit as well as active Fe protein. Extracts of such nifD or nifK deletion strains had no MoFe protein activity. However, active MoFe protein could be reconstituted by mixing extracts of the mutant strains. These results establish an approach for the purification of the individual MoFe protein subunits. Mutants lacking either or both of the MoFe protein subunits were still able to synthesize the iron-molybdenum cofactor (FeMo-cofactor), indicating that in A. vinelandii the FeMo-cofactor is preassembled and inserted into the MoFe protein. In contrast, a mutant strain lacking both the Fe protein and the MoFe protein failed to accumulate any detectable FeMo-cofactor. The further utility of specifically altered A. vinelandii strains for the study of the assembly, structure...Continue Reading


Jan 26, 1996·The Journal of Biological Chemistry·L PulakatN Gavini
Oct 29, 1998·The Journal of Biological Chemistry·E H Bursey, B K Burgess
Jul 5, 2011·Journal of Bacteriology·Trinity L HamiltonJohn W Peters
Nov 19, 2013·Journal of Bacteriology·César Poza-CarriónLuis M Rubio
Feb 9, 2016·Journal of Applied Microbiology·L J EberhartB M Barney
Feb 3, 2016·Scientific Reports·Drew MacKellarJürgen Prell
Apr 16, 2019·Frontiers in Microbiology·Mónica Navarro-RodríguezLuis M Rubio
Jan 1, 1990·Critical Reviews in Microbiology·S M Hinton, D Dean
Feb 7, 2001·The Journal of Biological Chemistry·J ChristiansenD R Dean
Jan 1, 2014·FEBS Letters·Emilio Jiménez-VicenteLuis M Rubio
Jan 18, 2018·Nature Microbiology·Yanning ZhengCaroline S Harwood
Mar 9, 2019·The Journal of Biological Chemistry·Emilio Jimenez-VicenteDennis R Dean
May 1, 1988·Molecular Microbiology·L T BennettD R Dean
Oct 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yilin HuM W Ribbe
Feb 5, 2016·Annual Review of Biochemistry·Yilin Hu, M W Ribbe
Oct 1, 1989·Molecular & General Genetics : MGG·M R JacobsonD R Dean
Jan 5, 2005·Journal of Bacteriology·Luis M Rubio, Paul W Ludden

Related Concepts

Gene Deletion Abnormality
CP2 nitrogenase
Partial Monosomy
Deletion Mutation
Glycoprotein Hormones, alpha Subunit
Purification Aspects
Structural gene

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.