Activity-Regulated Cytoskeleton-Associated Protein (Arc/Arg3.1) is Transiently Expressed after Heat Shock Stress and Suppresses Heat Shock Factor 1

Scientific Reports
A Young ParkKong-Joo Lee

Abstract

Heat shock proteins are induced by activation of heat shock factor 1 (HSF1) in response to heat shock and protect against heat stress. However, the molecular mechanisms underlying the downstream signal of heat shock have not been fully elucidated. We found that similarly to canonical Hsps, Arc/Arg3.1 is also markedly induced by heat shock and by other cellular stress inducers, including diamide, sodium arsenite and H2O2 in various cells. We noted that heat stress-induced Arc/Arg3.1 protein is short lived, with a half-life of <30 min, and is readily degraded by the ubiquitin-proteasome system. Arc/Arg3.1 overexpression inhibited the up-regulation of heat shock-induced Hsp70 and Hsp27, suggesting that Arc/Arg3.1 is a negative regulator of heat shock response (HSR). Studying the effect of Arc/Arg3.1 on HSF1, a major transcription factor in HSR, we found that Arc/Arg3.1 binds to HSF1 and inhibits its binding to the heat shock element in gene promoters, resulting in reduced induction of Hsp27 and Hsp70 mRNAs, without affecting HSF1's phosphorylation-dependent activation, or nuclear localization. Arc/Arg3.1 overexpression decreased cell survival in response to heat shock. We conclude that Arc/Arg3.1 is transiently expressed after hea...Continue Reading

References

Jan 1, 1986·Annual Review of Biochemistry·S Lindquist
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·W LinkD Kuhl
Jan 1, 1993·Annual Review of Biochemistry·J P Hendrick, F U Hartl
Sep 1, 1997·Molecular and Cellular Biology·D D MosserB Massie
Apr 16, 1998·Genes & Development·Y ShiR I Morimoto
Jan 6, 1999·Archives of Pharmacal Research·J E ParkC Kim
Jan 11, 2000·Molecular and Cellular Biology·E A NollenR I Morimoto
Dec 5, 2000·Brain Research·C Cirelli, G Tononi
Mar 12, 2002·The Journal of Biological Chemistry·Hee-Jung KimKong-Joo Lee
Apr 9, 2002·Molecular and Cellular Biochemistry·Hee-Jung Kim, Kong-Joo Lee
Sep 28, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Elhoucine MessaoudiClive R Bramham
Nov 14, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Clive R BramhamJohn F Guzowski
Aug 20, 2009·Experimental Brain Research·Clive R BramhamKarin Wibrand
May 11, 2010·The Journal of Biological Chemistry·Fuwang PengShilpa Buch
Jul 16, 2010·Nature Reviews. Molecular Cell Biology·Malin AkerfeltLea Sistonen
Jan 15, 2011·Science·Eran EdenUri Alon
Dec 2, 2011·Nature Reviews. Drug Discovery·Daniel W NeefDennis J Thiele
Aug 31, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yao LiuJing-Gen Liu
Jun 20, 2014·Neuron·Angela M MabbMichael D Ehlers
Jan 5, 2017·The FEBS Journal·Sharadha Dayalan Naidu, Albena T Dinkova-Kostova
May 10, 2017·Frontiers in Neuroscience·Stuart K Calderwood, Ayesha Murshid
Jul 4, 2017·Frontiers in Molecular Neuroscience·Agata GozdzJacek Jaworski
Sep 12, 2017·Trends in Cell Biology·Jian LiRichard I Morimoto
Sep 12, 2017·Seminars in Cell & Developmental Biology·Ruth E Carmichael, Jeremy M Henley
May 19, 2018·Cellular and Molecular Life Sciences : CMLS·János BarnaTibor Vellai
Oct 20, 2018·Current Cancer Drug Targets·Richard L Carpenter, Yesim Gökmen-Polar

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Citations

Sep 19, 2020·Scientific Reports·Kazuya KuboyamaMaki K Yamada
Jul 18, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Bingwei ZhangKe Tan

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Methods Mentioned

BETA
sumoylation
GTPase
transfection
immunoprecipitation
pull-down
confocal microscopy
PCR
ubiquitination
acetylation

Software Mentioned

xCelligence

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