Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5

Nature Communications
G Stefano BrigidiShernaz X Bamji

Abstract

Synaptic plasticity is mediated by the dynamic localization of proteins to and from synapses. This is controlled, in part, through activity-induced palmitoylation of synaptic proteins. Here we report that the ability of the palmitoyl-acyl transferase, DHHC5, to palmitoylate substrates in an activity-dependent manner is dependent on changes in its subcellular localization. Under basal conditions, DHHC5 is bound to PSD-95 and Fyn kinase, and is stabilized at the synaptic membrane through Fyn-mediated phosphorylation of a tyrosine residue within the endocytic motif of DHHC5. In contrast, DHHC5's substrate, δ-catenin, is highly localized to dendritic shafts, resulting in the segregation of the enzyme/substrate pair. Neuronal activity disrupts DHHC5/PSD-95/Fyn kinase complexes, enhancing DHHC5 endocytosis, its translocation to dendritic shafts and its association with δ-catenin. Following DHHC5-mediated palmitoylation of δ-catenin, DHHC5 and δ-catenin are trafficked together back into spines where δ-catenin increases cadherin stabilization and recruitment of AMPA receptors to the synaptic membrane.

References

Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·T TezukaT Yamamoto
Dec 22, 1999·Journal of Molecular Biology·N BlomS Brunak
Dec 2, 2000·Nature Neuroscience·E C BeattieR C Malenka
May 2, 2002·The Journal of Biological Chemistry·Tri-Hung NguyenPaul J Lombroso
May 3, 2002·Nature·Mitsutoshi SetouNobutaka Hirokawa
Apr 24, 2003·Science's STKE : Signal Transduction Knowledge Environment·Ali ZarrinparWendell A Lim
Jun 9, 2004·Nature Genetics·Jun MukaiJoseph A Gogos
Jun 23, 2004·American Journal of Human Genetics·M Daniele FallinAnn E Pulver
Dec 18, 2004·Neuron·Masaki FukataDavid S Bredt
Mar 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Alejandro Wolf-YadlinForest M White
Jun 8, 2007·Neuron·Chin-Yin TaiErin M Schuman
Aug 12, 2008·The European Journal of Neuroscience·Tomoko IsosakaShigeki Yuasa
Dec 19, 2008·Nature·Rujun KangAlaa El-Husseini
Feb 24, 2009·Progress in Lipid Research·Tsuyoshi IwanagaMasaki Fukata
Jul 15, 2009·The Journal of Cell Biology·Jun NoritakeMasaki Fukata
Feb 20, 2010·Nature Reviews. Neuroscience·Yuko Fukata, Masaki Fukata
Sep 28, 2010·Neuron·Antonio Sanz-ClementeKatherine W Roche
Apr 8, 2011·Molecular Biology of the Cell·Jennifer GreavesLuke H Chamberlain
Jul 20, 2011·The Journal of Physiology·Dong LiJohanna M Montgomery
Aug 9, 2011·FEBS Letters·Tarja KokkolaHans-Jürgen Kreienkamp
Nov 5, 2011·Trends in Neurosciences·Hiroshi OhnishiTakashi Matozaki

❮ Previous
Next ❯

Citations

Jun 1, 2016·Molecular Membrane Biology·Audrey Montersino, Gareth M Thomas
May 10, 2017·Experimental Biology and Medicine·Sabina TabaczarAleksander F Sikorski
Dec 15, 2017·Critical Reviews in Biochemistry and Molecular Biology·Sang Joon WonBrent R Martin
Jul 13, 2018·Critical Reviews in Biochemistry and Molecular Biology·María-Eugenia Zaballa, F Gisou van der Goot
Jun 21, 2017·Biochemical Society Transactions·David Tse Shen LinElizabeth Conibear
Jan 24, 2020·Molecular Membrane Biology·Julie M Philippe, Paul M Jenkins
Apr 1, 2020·International Journal of Molecular Sciences·Katarzyna KwiatkowskaAnna Ciesielska
Feb 10, 2018·Critical Reviews in Biochemistry and Molecular Biology·Jacqueline HowieWilliam Fuller
Apr 11, 2017·Frontiers in Plant Science·Yaxiao Li, Baoxiu Qi
Jan 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Oksana A Sergeeva, F Gisou van der Goot
Aug 14, 2019·EMBO Reports·Keith T Woodley, Mark O Collins
Feb 16, 2019·Frontiers in Molecular Neuroscience·Lucas MattJohannes W Hell
Feb 6, 2017·Journal of Neuroscience Research·Sabrina M Holland, Gareth M Thomas
Jul 13, 2017·The Biochemical Journal·Sabrina Chumpen RamirezJavier Valdez Taubas
Oct 16, 2018·Frontiers in Molecular Neuroscience·Alexandre DumoulinFritz G Rathjen
Mar 13, 2020·The Journal of Biological Chemistry·Yanling PanTheodore R Cummins
Feb 6, 2017·Journal of Experimental Botany·Piers A Hemsley
Jul 20, 2017·NPJ Breast Cancer·Alison M Anderson, Mark A Ragan
Jun 18, 2017·Biochemical Society Transactions·Kimon LemonidisJennifer Greaves
Jun 14, 2019·Journal of Cell Science·Bhavin S ShahShernaz X Bamji
Jul 14, 2020·Frontiers in Synaptic Neuroscience·Joseph P AlbanesiDavid M Jameson
Nov 15, 2018·Frontiers in Cellular Neuroscience·Brian G HiesterMatthew J Kennedy
Aug 21, 2020·The Journal of Biological Chemistry·Filip Zmuda, Luke H Chamberlain
Sep 25, 2019·Biophysical Journal·Jessica J ChenDarren Boehning
Jan 9, 2021·The FEBS Journal·Keith T Woodley, Mark O Collins
Jan 29, 2021·The FEBS Journal·Hening Lin
Nov 19, 2020·Journal of Cell Science·Christine SalaunLuke H Chamberlain
Jan 13, 2021·Molecular Brain·Benjun Ji, Małgorzata Skup
Apr 13, 2018·Molecular and Cellular Neurosciences·Ashley M BourkeMatthew J Kennedy
Mar 3, 2021·European Journal of Medicinal Chemistry·Sukriti SrivastavaSunil Kumar Khare
Feb 27, 2021·Journal of Molecular and Cellular Cardiology·Madeleine R MilesGea-Ny Tseng
Jun 1, 2020·Physiology & Behavior·Dianne P Figlewicz, Renger F Witkamp
Sep 28, 2020·Cellular Signalling·Caglar Gök, William Fuller
Apr 22, 2021·Open Biology·Martin Ian P Malgapo, Maurine E Linder
Mar 25, 2021·Journal of Cell Science·Jordan J ShimellShernaz X Bamji
May 14, 2021·Frontiers in Molecular Biosciences·Jessica J ChenDarren Boehning

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence recovery after photobleaching
immunoprecipitation
PCR

Software Mentioned

Photoshop
NetPhos
ImageJ
Fluoview
Illustrator CS6
GraphPad
Prism
Image J

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.