Activity staining of acylphosphatase after gel electrophoresis.

Analytical Biochemistry
Y MizunoH Shiokawa

Abstract

Acylphosphatase is an ubiquitous enzyme found in a variety of mammalian and avian tissues. Two isozymes of different amino acid sequence have been found in human, chicken, pig, and horse. To survey the distribution of the acylphosphatase isozymes among animal species and tissues, we have developed an activity staining procedure for the enzyme after electrophoresis on a polyacrylamide gel. Tissue extracts of pigs were subjected to electrophoresis, then the gel was stained for acylphosphatase activity in a solution containing acetyl phosphate and lead nitrate. Three activity bands were observed: the slowest moving one, which coincided with that of purified testis acylphosphatase, was widely distributed in testis, muscle, brain, heart, spleen, kidney, liver, and erythrocyte; the other two bands, which coincided with monomer and dimer of purified muscle acylphosphatase, were relatively localized in skeletal muscle.

References

Nov 1, 1987·Journal of Biochemistry·O MinowaH Shiokawa
Nov 1, 1987·Journal of Biochemistry·Y OhbaH Shiokawa
Dec 1, 1987·Journal of Biochemistry·H FujitaH Shiokawa
Aug 1, 1988·Journal of Protein Chemistry·G ManaoG Camici
Apr 1, 1985·Journal of Biochemistry·Y MizunoH Shiokawa
Apr 1, 1985·Journal of Biochemistry·T KizakiH Shiokawa
Sep 1, 1985·Archives of Biochemistry and Biophysics·G ManaoG Ramponi
Oct 1, 1985·Journal of Biochemistry·Y OhbaH Shiokawa
Jul 1, 1986·International Journal of Peptide and Protein Research·A BertiG Ramponi
Jul 1, 1966·Archives of Biochemistry and Biophysics·G RamponiA A Guerritore
Aug 1, 1984·Journal of Biochemistry·Y MizunoH Shiokawa
Dec 1, 1983·European Journal of Biochemistry·G CamiciG Ramponi
Apr 1, 1982·Archives of Biochemistry and Biophysics·E M Taga, R L Van Etten

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Citations

May 26, 2017·The Journal of Biological Chemistry·Moritz SengerBasem Soboh
Oct 16, 2002·The Journal of Biological Chemistry·Athanasios PaschosAugust Böck
May 1, 1990·Archives of Biochemistry and Biophysics·Y MizunoH Shiokawa

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