Actopaxin is phosphorylated during mitosis and is a substrate for cyclin B1/cdc2 kinase

The Biochemical Journal
Michael CurtisC E Turner

Abstract

Prior to cell division, normal adherent cells adopt a round morphology that is associated with a loss of actin stress fibres and disassembly of focal adhesions. In this study, we investigate the mitotic phosphorylation of the recently described paxillin and actin-binding focal-adhesion protein actopaxin [Nikolopoulos and Turner (2000) J. Cell Biol. 151, 1435-1448]. Actopaxin is comprised of an N-terminus containing six putative cdc2 phosphorylation sites and a C-terminus consisting of tandem calponin homology domains. Here we show that the N-terminus of actopaxin is phosphorylated by cyclin B1/cdc2 kinase in vitro and that this region of actopaxin precipitates cdc2 kinase activity from mitotic lysates. Actopaxin exhibits reduced electrophoretic mobility during mitosis that is dependent on phosphorylation within the first two consensus cdc2 phosphorylation sites. Finally, as cells progress from mitosis to G(1) there is an adhesion-independent dephosphorylation of actopaxin, suggesting that actopaxin dephosphorylation precedes cell spreading and the reformation of focal adhesions. Taken together, these results suggest a role for cyclin B1/cdc2-dependent phosphorylation of actopaxin in regulating actin cytoskeleton reorganization ...Continue Reading

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Citations

Nov 30, 2005·Cellular and Molecular Life Sciences : CMLS·J L Sepulveda, C Wu
Aug 28, 2012·Acta Pharmacologica Sinica·Si-Sheng Ou-YangHualiang Jiang
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Kyle R LegateReinhard Fässler
Sep 8, 2012·The Journal of Biological Chemistry·Jeanine PignatelliChristopher E Turner
Apr 23, 2010·Biointerphases·Gilles WederMartha Liley
Sep 9, 2004·The Journal of Cell Biology·Dominic M ClarkeChristopher E Turner
Mar 28, 2008·Journal of Cellular and Molecular Medicine·Cimona V HintonHava Karsenty Avraham
Jul 25, 2006·Current Biology : CB·David P LaLondeChristopher E Turner
Jul 13, 2004·Biochimica Et Biophysica Acta·Chuanyue Wu
Jan 5, 2010·Advances in Enzyme Regulation·Alessia ErricoTim Hunt
Jun 30, 2016·Nature Reviews. Molecular Cell Biology·Nitya Ramkumar, Buzz Baum
Jan 21, 2006·World Journal of Gastroenterology : WJG·Wei-Zhong WuZhao-You Tang
Dec 15, 2006·Journal of the American Society of Nephrology : JASN·Kyu Yong JungChuanyue Wu
Apr 9, 2005·The Journal of Biological Chemistry·David P LaLondeChristopher E Turner

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