ActuAtor, a molecular tool for generating force in living cells: Controlled deformation of intracellular structures

BioRxiv : the Preprint Server for Biology
H. NakamuraTakanari Inoue


Mechanical force underlies fundamental cell functions such as division, migration and differentiation. While physical probes and devices revealed cellular mechano-responses, how force is translated inside cells to exert output functions remains largely unknown, due to the limited techniques to manipulate force intracellularly. By engineering an ActA protein, an actin nucleation promoting factor derived from Listeria monocytogenes, and implementing this in protein dimerization paradigms, we developed a molecular tool termed ActuAtor, with which actin polymerization can be triggered at intended subcellular locations to generate constrictive force in a rapidly inducible manner. The ActuAtor operation led to striking deformation of target intracellular structures including mitochondria, Golgi apparatus, nucleus, and non-membrane-bound RNA granules. Based on functional analysis before and after organelle deformation, we found the form-function relationship of mitochondria to be generally marginal. The modular design and genetically-encoded nature enable wide applications of ActuAtor for studies of intracellular mechanobiology processes.

Related Concepts

Trees (plant)
Fixation - Action
Gene Polymorphism
Phylogenetic Analysis

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Journal of the American Chemical Society
Avishay PelahThomas M Jovin
The Review of Scientific Instruments
Pingqing Fan, Jianyu Liu
IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Adrien BadelTetsuaki Nakano
IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
K YaoL C Lim
© 2021 Meta ULC. All rights reserved