Acute 40 percent exchange-transfusion with hemoglobin-vesicles (HbV) suspended in recombinant human serum albumin solution: degradation of HbV and erythropoiesis in a rat spleen for 2 weeks

Transfusion
Hiromi SakaiKoichi Kobayashi

Abstract

Hemoglobin-vesicles (HbVs; diameter, 251 +/- 81 nm) are artificial O(2) carriers. Their efficacy for acute exchange transfusion has been characterized in animal models. However subsequent profiles of recovery involving the degradation of HbV in the reticuloendothelial system (RES) and hematopoiesis remain unknown. Isovolemic 40 percent exchange transfusion was performed in 60 male Wistar rats with HbV suspended in 5 g per dL recombinant human serum albumin (rHSA; HbV/rHSA, [Hb] = 8.6 g/dL), stored rat RBCs suspended in rHSA (sRBC/rHSA), or rHSA alone. Hematological and plasma biochemical analyses and histopathological examination focusing on the spleen were conducted for the subsequent 14 days. The reduced hematocrit (Hct) level (26%) for the HbV/rHSA and rHSA groups returned to its original level (43%) in 7 days. Plasma erythropoietin was elevated in all groups: the rHSA group showed the highest value on Day 1 (321 +/- 123 mIU/mL) relating to the anemic conditions (HbV/rHSA, 153 +/- 22; sRBC/rHSA, 63 +/- 7; baseline, 21 +/- 3). Simultaneously, splenomegaly occurred in all the groups as HbV/rHSA > rHSA > sRBC/rHSA. Histopathologically, the accumulated HbV in the spleen was undetectable by Day 14, but hemosiderin was deposited i...Continue Reading

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Citations

May 22, 2013·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Yuka SaitoYukihiro Kondo
Jan 31, 2009·Artificial Organs·Hiromi SakaiEishun Tsuchida
Mar 1, 1972·Transfusion·I L WoolfB M Babior
Nov 29, 2007·Journal of Internal Medicine·H SakaiE Tsuchida
Nov 9, 2006·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Eishun TsuchidaKoichi Kobayashi
Nov 21, 2007·Journal of Liposome Research·Hiromi Sakai, Eishun Tsuchida
Jul 25, 2009·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Yoshitaka YamanashiAtsuhiro Sakamoto
Mar 14, 2009·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Miki YamaguchiHisami Ikeda
Mar 16, 2017·BMC Anesthesiology·Yusuke NaitoMasahiko Kawaguchi
May 28, 2009·The Journal of Physical Chemistry. B·Takaaki SatoEishun Tsuchida
Feb 12, 2009·Bioconjugate Chemistry·Eishun TsuchidaKoichi Kobayashi
Jun 15, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Hiromi SakaiEishun Tsuchida

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