Acute action of choriogonadotropin on Leydig tumor cells: changes in the topography of the mitochondrial peripheral-type benzodiazepine receptor

Endocrinology
N BoujradV Papadopoulos

Abstract

We examined the topography of the MA-10 Leydig tumor cell mitochondrial peripheral-type benzodiazepine receptor (PBR). In previous studies, the 18 kDa PBR was found to be functionally associated with the voltage-dependent anion channel, located in the junctions between outer and inner membranes. Transmission electron (TEM) and atomic force microscopy (AFM) of immunogold labeled PBR on Leydig cell mitochondrial preparations showed that the 18 kDa PBR protein is organized in clusters of 4-6 molecules. Addition of hCG to Leydig cells induces a rapid, within 30 sec, increase in PBR ligand binding and morphological changes, namely redistribution of PBR molecules in large clusters (>7 particles). These hCG-induced changes were inhibited by a cAMP-dependent protein kinase inhibitor and by the benzodiazepine flunitrazepam. AFM further demonstrated the rapid reorganization of the mitochondrial membrane, where the formation of contacts between the outer and the inner mitochondrial membrane may facilitate cholesterol transfer.

Citations

Feb 7, 2007·Molecular and Cellular Endocrinology·Vassilios PapadopoulosMartine Culty
Mar 17, 2009·Biochimica Et Biophysica Acta·Malena B RoneVassilios Papadopoulos
Oct 16, 2015·Journal of Medicinal Chemistry·Rajeshwar NarlawarMichael Kassiou
Dec 15, 2010·Molecular and Cellular Endocrinology·Andrew MidzakVassilios Papadopoulos
Dec 19, 2012·Molecular and Cellular Endocrinology·Leeyah IssopVassilios Papadopoulos
Jun 15, 2010·Structure·Vladimir M KorkhovChristopher G Tate
Nov 22, 2012·Best Practice & Research. Clinical Endocrinology & Metabolism·Vassilios Papadopoulos, Walter L Miller
Jul 10, 2012·Biochimica Et Biophysica Acta·David TeboulJean-Jacques Lacapere
Dec 17, 2016·International Journal of Molecular Sciences·Tamara AzarashviliVassilios Papadopoulos
Apr 29, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·David R OwenChristine A Parker
Nov 22, 2014·Apoptosis : an International Journal on Programmed Cell Death·Barbara CostaClaudia Martini
Apr 15, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jun LiuVassilios Papadopoulos
Jan 26, 2005·Endocrine Research·V Papadopoulos
Sep 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zeineb Si ChaibGiulia Rossetti
Jan 13, 2004·Physiological Reviews·Kjetil Taskén, Einar Martin Aandahl
Jan 18, 2003·Endocrine Research·T HauetV Papadopoulos
May 16, 2019·Biochemical Society Transactions·Giulia RossettiPaolo Carloni
Nov 3, 2004·BioTechniques·Bradley E LaytonChia-Wei Wang
Oct 20, 2006·The Journal of Biological Chemistry·Jun LiuVassilios Papadopoulos
Jan 13, 2009·Journal of Medicinal Chemistry·Alana M ScarfMichael Kassiou
Dec 1, 1998·European Journal of Pharmacology·M Okubo, M Kawaguchi
Jul 27, 1999·The Journal of Steroid Biochemistry and Molecular Biology·M CultyV Papadopoulos

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