Acute and chronic effects of some dietary bioactive compounds on folic acid uptake and on the expression of folic acid transporters by the human trophoblast cell line BeWo

The Journal of Nutritional Biochemistry
Elisa KeatingFátima Martel

Abstract

Folic acid (FA) is a vitamin that acts as a coenzyme in the biosynthesis of purine and pyrimidine precursors of nucleic acids, which are critically important during pregnancy. Our group has previously shown that both reduced folate carrier (RFC1) and folate receptor alpha (FRalpha) seem to be involved in the uptake of [3H]folic acid ([3H]FA) by a human trophoblast cell line (BeWo) and by human primary cultured cytotrophoblasts. Our aim was to study the interaction between FA and some nutrients/bioactive substances. For this, we tested the acute and chronic effects of some dietary compounds on [3H]FA apical uptake and on the expression of both RFC1 and FRalpha mRNA in BeWo cells. Our results show that [3H]FA uptake was significantly reduced by acute exposure to epicatechin, isoxanthohumol (1-400 microM) or theophylline (0.1-100 microM); isoxanthohumol seemed to act as a competitive inhibitor, whereas epicatechin and theophylline caused an increase in both Km and Vmax. On the other hand, [3H]FA uptake was significantly increased by chronic exposure to xanthohumol, quercetin or isoxanthohumol (0.1-10 microM), and this increase does not seem to result from changes in the level of RFC1 or FRalpha gene expression. Moreover, [3H]FA up...Continue Reading

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Citations

Nov 22, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Pedro GonçalvesFátima Martel
Aug 10, 2007·The Journal of Membrane Biology·Elisa KeatingFátima Martel
Jun 29, 2013·Reproductive Toxicology·João Ricardo AraújoFátima Martel
Apr 16, 2010·Nutrition Research Reviews·Fátima MartelConceição Calhau
Mar 23, 2011·Nutrition Research·João R AraújoFátima Martel
Nov 8, 2014·Human Reproduction Update·Christina LyAndrée Gruslin
Jun 29, 2017·International Journal of Molecular Sciences·Chanchal MandalYoung Gyu Chai
Jul 6, 2017·Journal of Applied Toxicology : JAT·Chanchal MandalYoung Gyu Chai
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Oct 10, 2020·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Tong GuanXiujuan Zhao
Sep 4, 2021·Neurotoxicology· SushmaSrikanta Kumar Rath

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