Acute and specific impairment of spermatogonial development by GnRH antagonist-induced gonadotrophin withdrawal in the adult macaque (Macaca fascicularis)

Journal of Reproduction and Fertility
Y ZhengweiR I McLachlan

Abstract

This study examined the effect of GnRH-antagonist (GnRH-A)-induced gonadotrophin withdrawal on numbers of germ cells in adult cynomolgus monkeys and aimed to identify the site of the earliest spermatogenic lesion(s) produced. Animals received either GnRH-A (Cetrorelix; 450 micrograms kg-1 day-1 s.c.; n = 5) or vehicle (control, n = 4) for 25 days. One testis was removed on day 16 and the other testis on day 25. The optical disector stereological method was used to estimate germ and Sertoli cell numbers per testis. After GnRH-A treatment for 16 days, the number of type A spermatogonia was unchanged; however, type B spermatogonia (15% of control), preleptotene + leptotene + zygotene (15% control) and pachytene (55% control) spermatocytes were all reduced (P < 0.05). By day 25, these cells were further reduced together with step 1-6 spermatids (38% control; P < 0.05). More mature germ cells were unaffected. The proportion of type A pale spermatogonia at stages VII-XII was reduced (P < 0.05) in GnRH-A-treated groups (52% on day 16, 43% on day 25) compared with control (67%). After 25 days of GnRH-A treatment, the number of Sertoli cells was unaltered but nuclear volume was reduced (66% control, P < 0.05). Tubule length was unchange...Continue Reading

Citations

Jun 3, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Marvin L Meistrich, Gunapala Shetty
Apr 24, 2004·American Journal of Obstetrics and Gynecology·Christina Wang, Ronald S Swerdloff
Nov 11, 2011·The Veterinary Journal·G Garcia RomeroC Gobello
Dec 1, 2000·Baillière's Best Practice & Research. Clinical Endocrinology & Metabolism·R I McLachlan
Aug 16, 2013·Biology of Reproduction·Barbara MuciacciaElena Vicini
May 4, 2012·Toxicologic Pathology·Robert E Chapin, Dianne M Creasy
Dec 6, 2002·Endocrine Reviews·R A Anderson, D T Baird
Feb 10, 2006·Annals of the New York Academy of Sciences·Tony M PlantGary R Marshall
May 1, 2014·Spermatogenesis·Suresh Ramaswamy, Gerhard F Weinbauer
May 1, 2014·Spermatogenesis·Justin D Vidal, Katharine M Whitney

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