Acute Effect of Electronic Cigarette-Generated Aerosol From Flavored CBD-Containing Refill Solutions on Human Bronchial Epithelial Cells

Frontiers in Physiology
Noel J Leigh, Maciej L Goniewicz

Abstract

Introduction: Although electronic cigarettes (e-cigarettes) were originally developed to deliver aerosolized nicotine to lungs, recent data have shown that consumers also use them for inhalation of other drugs, including cannabidiol (CBD). The aim of this study was to test the acute inhalation toxicity of flavored CBD-containing aerosols emitted from e-cigarettes. Methods: Bronchial epithelial cells (H292) cells were exposed to aerosol generated from e-cigarettes refilled either with (1) propylene glycol solvent only (PG, control), (2) commercially purchased unflavored solution with CBD, or (3) commercially purchased solutions with and without CBD and with different flavors. The in vitro toxicological effects were assessed using the following methods: (1) trypan blue exclusion assay (cell viability), (2) neutral red uptake assay (metabolic activity), and (3) ELISA (concentrations of inflammatory mediators). Results: Most flavored products with or without CBD were cytotoxic as compared to the air control. Overall, aerosols with CBD were more cytotoxic than aerosols without CBD irrelevant of the flavoring used in the product. Although, unflavored aerosols containing CBD in PG were significantly more cytotoxic than aerosols contai...Continue Reading

References

Jan 18, 2016·Drug and Alcohol Dependence·Dustin C LeeAlan J Budney
Sep 14, 2016·Frontiers in Pharmacology·Michelle R PeaceAlphonse Poklis
Oct 6, 2017·Substance Abuse : Research and Treatment·Deric R KenneMark Banks
Dec 13, 2019·Addictive Behaviors Reports·Katrina F TriversGillian L Schauer
Aug 6, 2020·International Journal of Molecular Sciences·Daniela CerretaniLucia Micheli

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Citations

Oct 9, 2021·Chemical Research in Toxicology·Jiries Meehan-Atrash, Irfan Rahman

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