Acute effect of ethanol on membranes of the endoplasmic reticulum and on protein synthesis in rat liver

Alcoholism, Clinical and Experimental Research
C N MurtyH Sidransky

Abstract

The acute effect of ethanol on the membranes of hepatic endoplasmic reticulum, on the in vitro protein-synthetic activities of hepatic free and membrane-bound polyribosomes and on the plasma proteins of rats fasted overnight was investigated. Ethanol (0.75 g/100 g body weight) was tube-fed as a 50% (v/v) solution in saline 3 hr before sacrifice. Hepatic endoplasmic reticulum membranes from control and ethanol-treated rats were compared using the following techniques: (1) lactoperoxidase-catalyzed radioiodination of proteins and sodium dodecylsulfate-polyacrylamide gel electrophoresis, and (2) measurement of 14C-choline incorporation into membranes. Hepatic microsomal membranes from ethanol-treated rats incorporated in vitro les 125I into total proteins (as well as into the 55,000 molecular weight proteins) and incorporated in vivo less 14C-choline into microsomal membranes than membranes of control rats. Ethanol administration inhibited in vivo incorporation of 14C-leucine or 14C-phenylalanine into liver protein and plasma albumin and globulin. The data also indicate that an acute dose of ethanol reduced the in vitro protein-synthetic activity of hepatic membrane-bound polyribosomes, while free polyribosomes were relatively una...Continue Reading

References

Apr 25, 1975·Annals of the New York Academy of Sciences·C S Lieber
Jan 20, 1977·Biochimica Et Biophysica Acta·J Mørland, A Bessesen
Jul 1, 1977·Chemico-biological Interactions·H SidranskyM Reid
Jan 1, 1976·Annals of the New York Academy of Sciences·J A Thompson, R C Reitz
Aug 22, 1967·Biochimica Et Biophysica Acta·T StaehelinH Sidransky
Jul 1, 1971·The American Journal of Clinical Nutrition·H SidranskyD S Sarma
Nov 1, 1973·The American Journal of Clinical Nutrition·R E KirschS J Saunders
Sep 27, 1974·Biochimica Et Biophysica Acta·A PerinA Arnaboldi
Feb 1, 1974·Australian and New Zealand Journal of Medicine·T G ValeA Kelly
Feb 1, 1973·Canadian Journal of Biochemistry·D J TumaM F Sorrell
Feb 16, 1972·Biochemical and Biophysical Research Communications·R I Glazer, A C Sartorelli
Mar 1, 1972·The Biochemical Journal·K N JeejeebhoyU Sodtke
Sep 29, 1972·Biochimica Et Biophysica Acta·C N Murty, H Sidransky
Nov 19, 1971·Biochimica Et Biophysica Acta·S J Pilkis, A Korner
Aug 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·M C Ganoza, C A Williams
Nov 1, 1969·The Journal of Nutrition·S GaetaniM A Spadoni
Sep 1, 1971·The Journal of Clinical Investigation·M A RothschildS S Schreiber
Feb 22, 1971·Life Sciences. Pt. 2: Biochemistry, General and Molecular Biology·K KuriyamaG E Rauscher
Sep 1, 1966·Journal of Molecular Biology·H C Sox, M B Hoagland

❮ Previous
Next ❯

Citations

Jan 1, 1983·Pharmacology, Biochemistry, and Behavior·J MørlandB Wallin
Dec 8, 2000·Biochemical Pharmacology·M E ReillyV R Preedy
Jun 1, 1983·Experimental and Molecular Pathology·H SidranskyE Verney
Feb 1, 1986·British Journal of Haematology·R F LevineF Sieber
May 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G D VolentineM F Sorrell
Nov 1, 1981·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D J TumaM F Sorrell
Sep 28, 1984·Biochemical and Biophysical Research Communications·M E MailliardD J Tuma
Jan 1, 1982·Alcoholism, Clinical and Experimental Research·C N MurtyH Sidransky
Mar 1, 1984·Alcoholism, Clinical and Experimental Research·B WallinJ Mørland
Apr 21, 2005·Environmental Toxicology and Chemistry·Marla SmithwickRune Dietz
Jan 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D Schachter

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.