Acute effects of 17beta-estradiol on myocardial pH, Na+, and Ca2+ and ischemia-reperfusion injury

American Journal of Physiology. Cell Physiology
Steven E AndersonPeter M Cala

Abstract

Evidence suggests that 1) ischemia-reperfusion injury is due largely to cytosolic Ca(2+) accumulation resulting from functional coupling of Na(+)/Ca(2+) exchange (NCE) with stimulated Na(+)/H(+) exchange (NHE1) and 2) 17beta-estradiol (E2) stimulates release of NO, which inhibits NHE1. Thus we tested the hypothesis that acute E2 limits myocardial Na(+) and therefore Ca(2+) accumulation, thereby limiting ischemia-reperfusion injury. NMR was used to measure cytosolic pH (pH(i)), Na(+) (Na(i)(+)), and calcium concentration ([Ca(2+)](i)) in Krebs-Henseleit (KH)-perfused hearts from ovariectomized rats (OVX). Left ventricular developed pressure (LVDP) and lactate dehydrogenase (LDH) release were also measured. Control ischemia-reperfusion was 20 min of baseline perfusion, 40 min of global ischemia, and 40 min of reperfusion. The E2 protocol was identical, except that 1 nM E2 was included in the perfusate before ischemia and during reperfusion. E2 significantly limited the changes in pH(i), Na(i)(+), and [Ca(2+)](i) during ischemia (P < 0.05). In control OVX vs. OVX+E2, pH(i) fell from 6.93 +/- 0.03 to 5.98 +/- 0.04 vs. 6.96 +/- 0.04 to 6.68 +/- 0.07; Na(i)(+) rose from 25 +/- 6 to 109 +/- 14 meq/kg dry wt vs. 25 +/- 1 to 76 +/- 3; [...Continue Reading

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Citations

Jul 14, 2012·Cardiology Research and Practice·Masashi TawaYasuo Matsumura
Feb 18, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·S F PedersenP M Cala
Oct 14, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tepmanas Bupha-IntrR J Solaro
Jan 21, 2016·Journal of Cardiovascular Translational Research·Sivaporn SivasinprasasnNipon Chattipakorn
May 3, 2019·Acta Physiologica·Rosephine Del FernandesW Glen Pyle
Feb 12, 2011·Cellular and Molecular Neurobiology·Julio C SánchezLina De Los Reyes

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