Acute effects of cigarette smoke on three-dimensional cultures of normal human oral mucosa

Inhalation Toxicology
Alice GualerziElena Donetti

Abstract

Human oral mucosa is the combustion chamber of cigarette, but scanty evidence is available about the early smoke effects. The present work aimed at evaluating from a morphological point of view whole smoke early effects on epithelial intercellular adhesion and keratinocyte terminal differentiation in a three-dimensional model of human oral mucosa. Biopsies of keratinized oral mucosa of healthy nonsmoking women (n = 5) were collected. After culturing in a Transwell system, one fragment of each biopsy was exposed to the smoke of one single cigarette; the remnant represented the internal control. The distribution of epithelial differentiation markers (keratin-10, K10, and keratin-14, K14, for suprabasal and basal cells respectively), desmosomes (desmoglein-1, desmoglein-3), tight junctions (occludin), adherens junctions (E-cadherin, β-catenin), and apoptotic cells (p53, caspase 3) were evaluated by immunofluorescence. Quantitative analysis of K14 immunolabeling revealed an overexpression in the suprabasal layers as early as 3 h after smoke exposure, without impairment of the epithelial junctional apparatus and apoptosis induction. These results suggested that the first significant response to cigarette smoke came from the basal an...Continue Reading

References

Jul 13, 1999·Archives of Oral Biology·B ZappacostaB Giardina
Jan 2, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·R B Presland, B A Dale
Feb 24, 2001·Human & Experimental Toxicology·A RodgmanT A Perfetti
Aug 18, 2001·Skin Pharmacology and Applied Skin Physiology·J J Thiele
Oct 23, 2002·Journal of Clinical Periodontology·Gloria CalsinaJosé-Javier Echeverría
May 16, 2003·American Journal of Respiratory Cell and Molecular Biology·Julie A WickendenWilliam MacNee
Jun 16, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Nicole K ProiaJohn L Pauly
Jun 21, 2006·Free Radical Biology & Medicine·Luigi RodellaNader G Abraham
Jun 27, 2006·Journal of Cellular and Molecular Medicine·Isabella Dalle-DonneAldo Milzani
Sep 15, 2007·Biochimica Et Biophysica Acta·Andrea Hartsock, W James Nelson
Sep 26, 2007·International Journal of Cancer. Journal International Du Cancer·Sara GandiniPeter Boyle
Oct 16, 2007·The Journal of Investigative Dermatology·Carien M Niessen
Feb 14, 2008·European Journal of Clinical Nutrition·C L Fischer WalkerR E Black
May 20, 2008·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Anja ThielenLutz Müller
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Radhakrishna Rao
Jul 23, 2008·Molecular Cancer Research : MCR·Jean-Philippe CoppeAna Krtolica
Dec 20, 2008·Analytical Chemistry·Judit BartalisJan B Wooten
Jan 14, 2009·Cancer Prevention Research·Katrina SteilingAvrum Spira
Feb 25, 2009·Journal of Clinical Periodontology·Chi Pui WanEsmonde F Corbet
Mar 27, 2009·European Journal of Oral Sciences·Elena DonettiChiarella Sforza
Apr 14, 2009·Current Opinion in Oncology·Maria Paula Curado, Mia Hashibe
May 9, 2009·Journal of Anatomy·Hermann H Bragulla, Dominique G Homberger
May 29, 2009·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Aug 19, 2009·Antioxidants & Redox Signaling·Graziano ColomboIsabella Dalle-Donne
Dec 18, 2009·Chemico-biological Interactions·Philip C BurchamColin A Thompson
Feb 25, 2010·Cancer Prevention Research·Jay O BoyleAndrew J Dannenberg
Nov 26, 2010·Annual Review of Physiology·Jerome S Brody, Katrina Steiling
Jan 8, 2011·Head & Neck Oncology·Anna G ZygogianniVassilis Kouloulias
May 11, 2011·International Journal of Environmental Research and Public Health·Reinskje TalhoutAntoon Opperhuizen
May 21, 2011·Journal of Toxicology and Environmental Health. Part a·Abdelhabib SemlaliMahmoud Rouabhia

❮ Previous
Next ❯

Citations

Jul 16, 2013·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·David Thorne, Jason Adamson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Related Papers

Inhalation Toxicology
Julie St-LaurentElyse Bissonnette
Respirology : Official Journal of the Asian Pacific Society of Respirology
Weiyun ZhangHong Wei Chu
Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie
Kosuke OkuwaTomoki Nishino
Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie
Michaela Aufderheide, U Mohr
International Journal of Environmental Research and Public Health
Reinskje TalhoutAntoon Opperhuizen
© 2021 Meta ULC. All rights reserved