Acute Experimental Barrier Injury Triggers Ulcerative Colitis-Specific Innate Hyperresponsiveness and Ulcerative Colitis-Type Microbiome Changes in Humans.

Cellular and Molecular Gastroenterology and Hepatology
Jakob Benedict SeidelinJacob Tveiten Bjerrum

Abstract

The trigger hypothesis opens the possibility of anti-flare initiation therapies by stating that ulcerative colitis (UC) flares originate from inadequate responses to acute mucosal injuries. However, experimental evidence is restricted by a limited use of suitable human models. We thus aimed to investigate the acute mucosal barrier injury responses in humans with and without UC using an experimental injury model. A standardized mucosal break was inflicted in the sigmoid colon of 19 patients with UC in endoscopic and histological remission and 20 control subjects. Postinjury responses were assessed repeatedly by high-resolution imaging and sampling to perform Geboes scoring, RNA sequencing, and injury niche microbiota 16S ribosomal RNA gene sequencing. UC patients had more severe endoscopic postinjury inflammation than did control subjects (P < .01), an elevated modified Geboes score (P < .05), a rapid induction of innate response gene sets (P < .05) and antimicrobial peptides (P < .01), and engagement of neutrophils (P < .01). Innate lymphoid cell type 3 (ILC3) markers were increased preinjury (P < .01), and ILC3 activating cytokines were highly induced postinjury, resulting in an increase in ILC3-type cytokine interleukin-17A. ...Continue Reading

Associated Datasets

References

Jan 10, 2002·Gastroenterology·Alexander SwidsinskiHerbert Lochs
Mar 18, 2005·Annual Review of Immunology·Anthony W Segal
Mar 1, 2006·Lancet·Daniel J B MarksAnthony W Segal
Mar 30, 2012·Omics : a Journal of Integrative Biology·Guangchuang YuQing-Yu He
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Jul 16, 2014·The Journal of Experimental Medicine·Randy S LongmanDan R Littman
Nov 15, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jakob Benedict SeidelinOle Haagen Nielsen
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Jan 7, 2015·Cold Spring Harbor Perspectives in Medicine·Makoto TakeoMayumi Ito
Feb 26, 2015·Journal of Leukocyte Biology·Eric L Campbell, Sean P Colgan
Oct 22, 2015·American Journal of Respiratory and Critical Care Medicine·Roshell MuirRebecca J Ingram
Nov 26, 2015·Nucleic Acids Research·Guangshun WangZhe Wang
Jul 28, 2016·The Journal of Investigative Dermatology·Marie-Charlotte BrüggenGeorg Stingl
Feb 14, 2017·Nature Microbiology·Jonas HalfvarsonJanet K Jansson
Jun 10, 2017·Science·Sabine A EmingPaul Martin
Jul 12, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·David MasopustStephen C Jameson
Dec 13, 2017·Cold Spring Harbor Perspectives in Biology·Maho NagasawaXavier Romero Ros
Jan 4, 2018·Nature Communications·Michael SchubertJulio Saez-Rodriguez
Feb 1, 2018·Cell and Tissue Research·Jing Wang
Sep 29, 2018·F1000Research·Steven W Wingett, Simon Andrews
Jan 7, 2019·Mayo Clinic Proceedings·Guilherme Piovezani Ramos, Konstantinos A Papadakis
Jun 25, 2019·The American Journal of Gastroenterology·Natalie C KnoxCharles N Bernstein
Jul 23, 2019·The Journal of Clinical Investigation·Jennifer C BrazilCharles A Parkos
Jul 26, 2019·Nature Biotechnology·Evan BolyenJ Gregory Caporaso
Jul 30, 2019·Frontiers in Immunology·Jessica G BorgerMargaret L Hibbs
Oct 12, 2019·Expert Review of Gastroenterology & Hepatology·Eduard F Stange, Bjoern O Schroeder
Apr 1, 2020·International Journal of Experimental Pathology·Daniela S Masson-MeyersMarco Andrey C Frade
Jul 11, 2020·Trends in Immunology·Wenqing Zhou, Gregory F Sonnenberg
Sep 12, 2020·Nature Reviews. Disease Primers·Taku KobayashiToshifumi Hibi
Oct 24, 2020·Journal of Pediatric Gastroenterology and Nutrition·Richard Kellermayer, Matthias Zilbauer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.