Acute glucose overload abolishes Ca2+ oscillation in cultured endothelial cells from bovine aorta: a possible role of superoxide anion

Circulation Research
C KimuraY Ito

Abstract

Effects of acute glucose overload on [Ca2+]i were investigated in cultured endothelial cells from bovine aorta. Application of 0.1 micromol/L ATP elicited an oscillatory increase in [Ca2+]i (Ca2+ oscillation) in Krebs solution containing 11.5 mmol/L glucose. The frequency of Ca2+ oscillation induced by ATP increased in a concentration-dependent manner, ranging between 0.03 and 1 micromol/L. When cells were preincubated with 23 mmol/L glucose-containing Krebs solution (high glucose solution) for 3 hours, 0.1 micromol/L ATP failed to induce Ca2+ oscillation but evoked only a phasic followed by sustained increase in [Ca2+]i. Application of a higher concentration of ATP (10 micromol/L) evoked a transient increase in [Ca2+]i both in control and high glucose-treated cells. However, the falling phase of [Ca2+]i was prolonged in high glucose-treated cells. Thapsigargin (1 micromol/L), an inhibitor of endoplasmic Ca2+-ATPase, induced a transient followed by a sustained increase in [Ca2+]i in control cells. Preincubation with high glucose solution increased the rate of rise of the thapsigargin-induced increase in [Ca2+]i and abolished the sustained increase, suggesting that glucose overload accelerates Ca2+ leak from intracellular store ...Continue Reading

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Citations

Jul 3, 2008·Pflügers Archiv : European journal of physiology·Tetsuya KoyamaMasahiro Oike
Aug 5, 2000·Free Radical Biology & Medicine·K M LounsburyR C Ziegelstein
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