Acute iodine deficiency induces a transient VEGF-dependent microvascular response in mammary glands involving HIF-1, ROS, and mTOR

American Journal of Physiology. Cell Physiology
J VanderstraetenAnne-Catherine Gérard

Abstract

Iodine deficiency (ID), which affects almost two billion people worldwide, is associated with breast pathologies such as fibrosis in human and induces breast atypia in animal models. Because ID induces vascular activation in the thyroid, another iodide-uptaking organ, and as breast is also sensitive to ID, we aimed to characterize ID-induced effects on the breast microvasculature in vivo and in two different breast cell lines in vitro. Virgin and lactating NMRI mice received an iodide-deficient diet and a Na+/I- symporter inhibitor for 1 to 20 days. Some virgin mice were treated with vascular endothelial growth factor A (VEGF) or VEGF receptor inhibitors. In vitro, ID was induced in MCF7 and MCF12A cells by replacing the iodide-containing medium by an iodide-deficient medium. In vivo, VEGF expression was increased following ID in mammary tissues. Consequently, ID induced a transient increase in mammary gland blood flow, measured after anesthesia, in virgin and lactating mice, which was repressed by VEGF or VEGF receptor inhibitors. In MCF7 cells, ID induced a transient increase in reactive oxygen species, followed by an increase in hypoxia-inducible factor-1α (HIF-1α) protein and VEGF mRNA expression. Antioxidant N-acetylcystei...Continue Reading

References

Nov 17, 1993·Journal of the National Cancer Institute·R G ZieglerM B Hyer
Aug 1, 1993·The American Journal of Physiology·D D KuT A Brock
Sep 1, 1996·Molecular and Cellular Biology·J A ForsytheG L Semenza
Jun 1, 1996·Journal of Cardiovascular Pharmacology·R YangH Jin
Aug 17, 1999·International Journal of Cancer. Journal International Du Cancer·P PisaniJ Ferlay
Oct 20, 1999·European Journal of Endocrinology·L VayreJ M Bidart
Mar 10, 2000·Cancer Causes & Control : CCC·S A CannC van Netten
Mar 17, 2000·The Journal of Clinical Endocrinology and Metabolism·M T KilbaneP P Smyth
May 18, 2000·Cell Biochemistry and Function·R WinklerW Wonisch
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·T KogaiG A Brent
Aug 18, 2000·The Journal of Clinical Endocrinology and Metabolism·J Y ChoS M Jhiang
Jun 8, 2002·The Journal of Biological Chemistry·Jennifer S IsaacsLeonard M Neckers
Oct 22, 2002·American Journal of Physiology. Endocrinology and Metabolism·James A Rillema, Melissa A Hill
Oct 9, 2003·The EMBO Journal·Nick R LeslieC Peter Downes
Feb 18, 2004·The Breast : Official Journal of the European Society of Mastology·S Venturi
Nov 15, 2006·Molecular and Cellular Biology·Satomi NadanakaKazutoshi Mori
Dec 13, 2006·Endocrine-related Cancer·O Arroyo-HelgueraC Aceves
Feb 16, 2008·The American Journal of Pathology·Anne-Catherine GérardFabrice Soncin
Jul 23, 2008·International Journal of Medical Sciences·Frederick R StoddardGregg J Johannes
Apr 2, 2009·American Journal of Physiology. Endocrinology and Metabolism·Anne-Catherine GérardIdes M Colin
Oct 2, 2009·Journal of Endocrinological Investigation·M SponzielloD Russo
Jun 18, 2010·Kidney International·Oscar Alvarez-GarcíaFernando Santos
Jul 23, 2011·Cell Death and Differentiation·H KatoM Kitamura
Oct 31, 2012·PloS One·Chantal EthierGuy G Poirier
Mar 12, 2013·Thyroid : Official Journal of the American Thyroid Association·Elizabeth N PearceMichael B Zimmermann
Jul 6, 2014·Physiological Reviews·Dmitry B ZorovSteven J Sollott
Mar 8, 2016·International Journal of Molecular Sciences·Hafiz Maher Ali ZeeshanHan-Jung Chae
Jul 26, 2017·Annals of Surgery·Jérôme DuisitBenoît Lengelé

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