Acute local subcutaneous VEGF165 injection for augmentation of skin flap viability: efficacy and mechanism

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Asim KhanCho Y Pang

Abstract

Distal skin ischemic necrosis is a common complication in skin flap surgery. The pathogenesis of skin flap ischemic necrosis is unclear, and there is no clinical treatment available. Here, we used the 4 x 10 cm rat dorsal skin flap model to test our hypothesis that subcutaneous injection of vascular endothelial growth factor 165 (VEGF165) in skin flaps at the time of surgery is effective in augmentation of skin flap viability, which is associated with an increase in nitric oxide (NO) production, and the mechanism involves 1) an increase in skin flap blood flow in the early stage after surgery and 2) enhanced angiogenesis subsequently to sustain increased skin flap blood flow and viability. We observed that subcutaneous injection of VEGF165 in skin flaps at the time of surgery increased skin flap viability in a dose-dependent manner. Subcutaneous injection of VEGF165 at the dose of 2 microg/flap increased skin flap viability by 28% (P < 0.05; n = 8). Over 80% of this effect was blocked by intramuscular injection of the NO synthase (NOS) inhibitor Nomega-nitro-L-arginine (13 mg/kg) 45 min before surgery (P < 0.05; n = 8). The VEGF165 treatment also increased skin flap blood flow (2.68 +/- 0.63 ml x min(-1) x 100 g(-1)) compared w...Continue Reading

References

Feb 1, 1978·Plastic and Reconstructive Surgery·J W MayJ V Hurley
Sep 1, 1992·Canadian Journal of Physiology and Pharmacology·M L KreidsteinN Xu
Apr 1, 1972·Plastic and Reconstructive Surgery·S H Milton
May 1, 1972·Plastic and Reconstructive Surgery·W C Grabb
Jun 2, 1967·Science·H Selye
Jun 1, 1982·Plastic and Reconstructive Surgery·C L Kerrigan, R K Daniel
Feb 1, 1980·Plastic and Reconstructive Surgery·G H Sasaki, C Y Pang
Dec 1, 1980·Current Problems in Surgery·J W May, G G Gallico
Jan 1, 1996·The American Journal of Physiology·L MorbidelliM Ziche
Apr 8, 1998·The American Journal of Physiology·J D HoodH J Granger
Feb 1, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·A PetterssonH F Dvorak
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·Y T XuanR Bolli
Jan 11, 2002·British Journal of Plastic Surgery·G O'TooleM Poole
Jul 5, 2003·The Journal of Surgical Research·Hans-Günther MachensPeter Mailänder
Feb 1, 1965·Plastic and Reconstructive Surgery·R M MCFARLANER A HENRY
Dec 3, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Homa AshrafpourCho Y Pang

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Citations

Feb 26, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Bernadette FerraroRichard Heller
May 4, 2010·Journal of Rehabilitation Research and Development·John A RussellGregory K Hartig
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Arnaud LacombeEric Vilain
Jul 4, 2012·Indian Journal of Plastic Surgery : Official Publication of the Association of Plastic Surgeons of India·Spyridon A VourtsisMihai Ionac
Jan 26, 2012·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Spyridon A VourtsisApostolos E Papalois
Jan 19, 2016·Journal of Tissue Engineering and Regenerative Medicine·Martina MoritzMartijn van Griensven
Jan 25, 2011·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Ovunc AkdemirEcmel Songur
Oct 7, 2008·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Mohammed Ali AkhavaniNorbert Kang
Aug 5, 2014·Veterinary and Comparative Orthopaedics and Traumatology : V.C.O.T·M KarayannopoulouD Dionyssiou
Nov 19, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Won Jai LeeDong Kyun Rah
Dec 3, 2014·Acta Biomaterialia·A LoweK J Balkus
Apr 27, 2017·Plastic and Reconstructive Surgery·S Morteza Seyed JafariAmiq Gazdhar
Apr 7, 2009·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Alper CelikSuna Omeroglu
Jul 27, 2005·Microsurgery·Jeff S IsenbergDavid D Roberts
Dec 5, 2014·Journal of Korean Medical Science·Dong Kyun RahWon Jai Lee
Aug 5, 2017·The Journal of Membrane Biology·S Morteza Seyed JafariRobert E Hunger
Aug 24, 2005·Antioxidants & Redox Signaling·Viren PatelChandan K Sen
Apr 6, 2006·The Laryngoscope·Kristopher HartJose Pizarro
Jun 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Wolfgang MichlitsSeyedhossein Aharinejad
Apr 20, 2019·Aesthetic Surgery Journal·Yizuo CaiWei Li
Jun 28, 2019·Journal of Plastic Surgery and Hand Surgery·Fatih Burak EfeoğluMetin Gorgu
May 9, 2015·Annals of Plastic Surgery·Dong Won LeeWon Jai Lee
Feb 28, 2014·Plastic and Reconstructive Surgery·Minekatsu Akimoto, Akira Takeda

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