Acute pancreatitis induces cytokine production in endotoxin-resistant mice

Annals of Surgery
J W EubanksS M Goyert

Abstract

The purpose of this study was to determine whether pathologic progression and cytokine responses in acute pancreatitis (AP) are altered in the absence of endotoxemia. Previous studies have demonstrated that AP is characterized by rapid production and release of inflammatory cytokines, which play a major role in the local pancreatic and systemic complications of this disease. Infection and endotoxemia have been implicated as a major source of morbidity and death in AP and as possible stimuli for the overwhelming cytokine response seen in this disease. AP was induced by a choline-deficient and ethionine-supplemented diet for 4 days in normal C57BL/6J mice (controls, n = 23) and in CD14 knockout mice (CD14KO, n = 23), which cannot produce circulating cytokines in response to endotoxin. Control and endotoxin-resistant mice were killed at time 0, then at 24, 48, 72, and 96 hours after the start of the diet. At each time point serum was collected for amylase, glucose, and cytokine measurements (tumor necrosis factor-alpha [TNFalpha] and interleukin-1beta [IL1beta]), and the pancreas was removed for histologic examination. TNFalpha was measured with a bioassay using WEHI-2F cells and IL1beta with a bioassay using D10.G4.1 cells. CD14K...Continue Reading

References

Dec 15, 1991·Journal of Immunological Methods·R S Wallis
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Jan 1, 1993·Methods in Enzymology·C L Stewart
Dec 1, 1995·The Journal of Surgical Research·C B HughesA O Gaber

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Citations

Mar 15, 2013·Canadian Journal of Surgery. Journal Canadien De Chirurgie·Akan YeniceriogluBurhan H Kanat
Dec 7, 2007·The Surgical Clinics of North America·Mohammed ElfarA Osama Gaber
Dec 24, 2005·Shock·Jill Granger, Daniel Remick
Feb 6, 2009·Scandinavian Journal of Gastroenterology·Hongxiang LiuZongguang Zhou
Aug 13, 2005·World Journal of Gastroenterology : WJG·Hong-Li JiangJu-Lin Gao

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