Acute parvovirus B19 infection frequently causes false-positive results in Epstein-Barr virus- and herpes simplex virus-specific immunoglobulin M determinations done on the Liaison platform.

Clinical and Vaccine Immunology : CVI
Mario Berth, Eugene Bosmans

Abstract

During an outbreak of parvovirus B19 we collected serum samples from 68 nonpregnant patients in the region of Antwerp (Belgium). Fifty-seven (84%) of the parvovirus B19 immunoglobulin M (IgM)-positive sera had a positive result for Epstein-Barr virus (EBV) IgM by Liaison testing, 61 (90%) had a positive result for herpes simplex virus (HSV) IgM, 20 (29%) samples were positive for cytomegalovirus IgM, and 15 (22%) had a positive result for Borrelia burgdorferi sensu lato IgM. As assay interference was suspected, sera were further investigated by using additional infectious-disease serology tests and by performing various interference elimination procedures. We could show that the EBV IgM and HSV IgM results were false positives due to aspecific IgM reactions with the solid phase. All samples were also analyzed by a modified Liaison EBV IgM assay, based on the addition of polyvinylpyrrolidone and polyvinyl alcohol to the dilution buffer, which partially eliminated this type of assay interference. Although the Liaison is a very convenient, automated immunoassay platform, this study demonstrates the potential for improvement of mainly the EBV IgM and HSV IgM tests.

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Citations

Mar 1, 2013·Journal of the Pediatric Infectious Diseases Society·Charles R Woods
Jul 13, 2012·Clinical and Vaccine Immunology : CVI·Nele WellinghausenUrsula Weber
May 2, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M BerthC Vauloup-Fellous
Dec 15, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M BerthN Christensen
Mar 31, 2012·Journal of Adolescence·Abigail MillingsPaul Stallard
May 8, 2015·Journal of Medical Virology·Seyed Mahmood Seyed KhorramiNazanin Zahra Shafiei Jandaghi
Mar 30, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·E K AlidjinouD Hober
Feb 12, 2010·Clinical and Vaccine Immunology : CVI·Mario Berth, Eugene Bosmans
Oct 24, 2014·Journal of Pediatric Gastroenterology and Nutrition·Kathleen B SchwarzUNKNOWN Pediatric Acute Liver Failure Study Group

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