PMID: 7539335Feb 1, 1995Paper

Acute phase-dependent changes in the binding of rat liver nucleoproteins to the cytokine response element of the rat haptoglobin gene

The International Journal of Biochemistry & Cell Biology
L SevaljevićM Petrović

Abstract

Hormones released during the acute phase reaction promote the transcriptional activation of the haptoglobin (Hp) gene and a consequent increase of Hp protein synthesis in the liver. The mechanisms underlying the alterations of basal transcription rates of eukaryotic genes are assumed to result from modulations of the binding affinities between nucleoproteins and specific DNA sequences in the enhancer and promoter elements. In order to characterize the changes in the interaction of nucleoproteins with the promoter that accompany the induction of the Hp gene, nuclear extracts from normal and inflamed livers were probed with hormone responsive element (HRE) of the rat Hp gene by gel mobility shift and Southwestern assays. Each of the three cis-acting sequences of the HRE, elements A, B, and C, recognized a distinct set of proteins. Together they conferred an additional level of specificity to the protein binding sites of the entire ABC-element. These sites were recognized by proteins in liver nuclear extracts isolated from both control and treated rats. The differences in the gel shift and Southwestern patterns of the corresponding DNA-protein complexes suggested that transcriptional activation of the Hp gene relied on changes in ...Continue Reading

References

Dec 1, 1992·The International Journal of Biochemistry·W M Krajewska
Aug 7, 1992·Cell·T Hunter, M Karin
Jan 1, 1989·Annals of the New York Academy of Sciences·I KushnerD Schultz
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D Z FisherG Blobel
Jan 11, 1980·Nucleic Acids Research·B BowenH Weintraub

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Citations

May 7, 2002·Cell Biology International·Desanka BogojevićMirjana Mihailović
Nov 23, 2012·Journal of Neuroscience Research·Shuguang YangShaojun Liu
Jun 13, 2009·Journal of Cellular Biochemistry·Melita VidakovićGoran Poznanović

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