Acute-phase gene expression correlates with intrahepatic tumor necrosis factor-alpha abundance but not with plasma tumor necrosis factor concentrations during sepsis/systemic inflammatory response syndrome in the rat

Critical Care Medicine
K M Andrejko, C S Deutschman

Abstract

To test the hypothesis that after cecal ligation and puncture in the rat, there is increased expression of the tumor necrosis factor (TNF)/interleukin-1-dependent, acute-phase reactant alpha 1-acid glycoprotein in the liver, and that this change correlates temporally with increased abundance of TNF-alpha in the hepatic parenchyma but not with circulating concentrations of TNF-alpha. Prospective, randomized, controlled study. Research laboratory at the University of Pennsylvania School of Medicine. Male, adolescent Sprague-Dawley rats, weighing 200 to 300 g. The procedure of cecal ligation and single puncture with an 18-gauge needle was performed in one group of animals. Control animals underwent sham operation. At 0, 3, 6, 16, 24, 48, and 72 hrs after either procedure, blood was collected and the liver was isolated and perfusion-fixed with 2% paraformaldehyde. In a second group of animals, liver tissue was harvested for isolation of total hepatic RNA. Northern blot hybridization analysis demonstrated an increase in steady-state concentrations of alpha 1-acid glycoprotein messenger RNA that peaked at 16 hrs after cecal ligation and puncture. The alpha 1-acid glycoprotein messenger RNA was not detected in control animals. TNF-alp...Continue Reading

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Citations

May 26, 1999·Infectious Disease Clinics of North America·J Carlet
Nov 14, 1997·Nature Medicine·G E Grau, D N Maennel
Jul 1, 1997·Critical Care Medicine·C S Deutschman
Nov 21, 1997·Critical Care Medicine·R P Dellinger
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