Acute-phase glycoprotein N-glycome of ovarian cancer patients analyzed by CE-LIF

Electrophoresis
Stefan WeizVéronique Blanchard

Abstract

Epithelial ovarian cancer (EOC) is the most frequent cause of death from all gynecological malignancies because of its late diagnosis. As N-glycosylation is modified in the course of ovarian cancer, it is a promising source of tumor biomarkers. In this work, serum glycoproteins, depleted from albumin and IgG, were separated by 2DE. Protein spots of acute-phase proteins were identified by peptide mapping and their corresponding glycan moieties were released enzymatically, fluorescently labeled and analyzed by CE-LIF. In the positive acute-phase proteins, haptoglobin, α1-antitrypsin, and α1-antichymotrypsin, an increase of antennarity and Lewis(X) motif could be measured in EOC patients on tri- and/or tetraantennary N-glycans. Tetraantennary N-glycans containing three Lewis(X) epitopes and triantennary N-glycans containing a β(1-6) branch and a Lewis(X) epitope were only present in EOC patients. We also showed for the first time that the core-fucosylated biantennary digalactosylated N-glycan of α1-acid glycoprotein is a potential biomarker for EOC. To conclude, core-fucosylated biantennary N-glycans on α1-acid glycoprotein as well as higher antennarity and increased amounts of Lewis(X) motif on haptoglobin, α1-antitrypsin, and α1...Continue Reading

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Citations

Jul 29, 2017·Electrophoresis·Mengmeng WangChunfang Gao
Aug 24, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Mengmeng WangChunfang Gao
Nov 2, 2018·Expert Review of Proteomics·Wenjing PengYehia Mechref
Oct 20, 2019·Molecular & Cellular Proteomics : MCP·Marton Szigeti, Andras Guttman
Oct 24, 2019·Current Molecular Medicine·Zsuzsanna ElekZsolt Rónai
Aug 11, 2018·Oncotarget·Stefan W de VroomeManfred Wuhrer
Jun 2, 2020·Frontiers in Immunology·Marta WieczorekVéronique Blanchard
Jan 7, 2021·Omics : a Journal of Integrative Biology·Medi KoriKazim Yalcin Arga
Dec 1, 2019·Molecular & Cellular Proteomics : MCP·Marton Szigeti, Andras Guttman
May 1, 2021·Diagnostics·Francis Mugeni Wanyama, Véronique Blanchard
Mar 13, 2018·Chemical Reviews·Grace LuLisa A Holland
Jan 21, 2017·Analytical Chemistry·Stefan GaunitzMilos V Novotny

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