Acute phase response to recombinant interleukin-6 and macrophage- and fibroblast-derived crude cytokine preparations in primary cultures of mouse hepatocytes.

Cell Biochemistry and Function
H RokitaA Koj

Abstract

A number of acute phase proteins were determined by electroimmunoassay in media from CBA mouse hepatocytes cultured for 2 days with human recombinant IFN beta 2/IL-6, as well as with conditioned media from LPS-stimulated rat macrophages, and of murine L fibroblasts. It was found that human recombinant IL-6 caused three-fold increase in secretion of fibrinogen, while haptoglobin, complement C3 and transferrin were increased respectively, to 168 per cent, 151 per cent, and 145 per cent of the control. DEX(10(-7) M) in DMEM supplemented with 5 per cent FCS, enhanced the IL-6 effect on the three positive acute phase proteins. IL-6 elevated haptoglobin mRNA in mouse hepatocytes to a degree comparable with the concentration of the protein in the culture medium. The effect of conditioned media from murine fibroblasts and peritoneal rat macrophages was generally similar to that of recombinant IL-6. However, both natural preparations of the cytokines caused decrease in albumin and alpha-1-proteinase inhibitor secretion.

References

Apr 1, 1986·Archives of Biochemistry and Biophysics·H BaumannM D Glibetic
Nov 1, 1986·The Journal of Clinical Investigation·D H PerlmutterH R Colten
May 1, 1988·European Journal of Immunology·T GeigerP C Heinrich
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·B Weeke
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas

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Citations

Feb 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H Baumann
Aug 12, 2005·Magnetic Resonance in Medical Sciences : MRMS : an Official Journal of Japan Society of Magnetic Resonance in Medicine·Akira UchinoSho Kudo

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