Acute regulation of steady-state GABA levels following GABA-transaminase inhibition in rat cerebral cortex

Neurochemistry International
Robin A de GraafK L Behar

Abstract

Cellular GABA levels are determined by the dynamic balance between synthesis and catabolism and are regulated at the level of glutamate decarboxylase, precursor availability (e.g., glutamate and glutamine), and possibly GABA degradation. GABA levels rise and stabilize within hours in human cortex following orally administered vigabatrin, an irreversible inhibitor of GABA-T, suggesting potential product inhibition of GABA synthesis or enhanced GABA degradation through the non-inhibited GABA-T fraction. In this study time courses of the rise in cortical GABA were measured in anesthetized rats in vivo after vigabatrin treatment using localized (1)H magnetic resonance spectroscopy and the times to reach steady-state for a given dose were determined. Rates of GABA synthesis were estimated for the period of constant GABA level from the accumulation of [2-(13)C]GABA following a short intravenous infusion (20 min) of either [1,6-(13)C(2)]glucose or [2-(13)C]acetate. No evidence of product inhibition of glutamate decarboxylase by the increased GABA concentration or reduced synthesis from [1,6-(13)C(2)]glucose (control, 0.031+/-0.010; vigabatrin-treated, 0.037+/-0.004 micromol/g/min, P=0.30) or [2-(13)C]acetate (control, 0.078+/-0.010; v...Continue Reading

References

Nov 1, 1990·Neurochemical Research·O M Larsson, A Schousboe
Nov 1, 1989·Journal of Neurophysiology·D A McCormick
Apr 1, 1985·Physics in Medicine and Biology·A HaaseD Matthaei
Nov 1, 1973·Journal of Neurochemistry·U PonténB K Siesjö
Jun 1, 1994·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·K L Behar, D Boehm
Dec 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·S W Provencher
Jun 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·R Gruetter
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D L RothmanR H Mattson
Oct 1, 1996·Journal of Neurochemistry·N E Preece, S Cerdán
Nov 26, 2002·Annals of Neurology·Lucien M LevyLeonardo G Cohen
Jul 31, 2003·Molecular Psychiatry·P BrambillaJ C Soares
Apr 2, 2005·Nature Reviews. Neuroscience·David A LewisDavid W Volk
Apr 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Anant B PatelKevin L Behar
May 21, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jehoon YangJun Shen

❮ Previous
Next ❯

Citations

Jan 16, 2007·Journal of the Association for Research in Otolaryngology : JARO·Thomas J BrozoskiCarol A Bauer
Jan 11, 2008·Epilepsia·Pasquale ParisiPaolo Curatolo
Dec 1, 2015·Neurochemistry International·Antero SalminenMikko Hiltunen
Jul 13, 2007·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·Pasquale ParisiPaolo Curatolo
Nov 11, 2008·Annals of the New York Academy of Sciences·Frederico C PereiraTice R Macedo
Feb 8, 2014·Computers in Biology and Medicine·Damanpreet SinghRajesh Kumar Goel
Apr 4, 2014·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Conny F WaschkiesBasil Künnecke
Mar 11, 2020·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Antoine CherixRolf Gruetter
Sep 6, 2019·Psychopharmacology·Alice Egerton
Jan 23, 2021·Environmental Science and Pollution Research International·Shimaa M Abou-ZeidHuda O AbuBakr

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.