Acute renal response to LPS: impaired arginine production and inducible nitric oxide synthase activity

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Karen A MungerMark J Lortie

Abstract

We have previously shown in rats that lipopolysaccharide (LPS) causes both decreased renal perfusion and kidney arginine production before nitric oxide (NO) synthesis, resulting in a >30% reduction in plasma arginine. To clarify the early phase effects of LPS, we asked the following two questions: 1) is the rapid change in renal arginine production after LPS simply the result of decreased substrate (i.e., citrulline) delivery to the kidney or due to impaired uptake and conversion and 2) is the systemic production of NO limited by plasma arginine availability after LPS? Arterial and renal vein plasma was sampled at 30-min intervals from anesthetized rats with or without citrulline or arginine (2 micromol.min(-1).kg(-1) iv) a dose with no effect on MAP, renal function, or NO production. Exogenous citrulline was quickly converted to arginine by the kidney, resulting in plasma levels similar to equimolar arginine infusion. Also, the increase in citrulline uptake resulted primarily from increased filtered load and reabsorption. In a separate series, citrulline was infused after LPS administration, verifying that citrulline uptake and conversion persists during impaired kidney function. Last, in rats given LPS, the elevation of plasm...Continue Reading

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Citations

Jul 16, 2011·International Journal of Radiation Biology·Andrea BabicovaJaroslav Chladek
Nov 13, 2009·American Journal of Physiology. Renal Physiology·Gin-Fu Chen, Chris Baylis

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