Acute Response of the Hippocampal Transcriptome Following Mild Traumatic Brain Injury After Controlled Cortical Impact in the Rat

Journal of Molecular Neuroscience : MN
Babru B SamalLee E Eiden

Abstract

We have previously demonstrated that mild controlled cortical impact (mCCI) injury to rat cortex causes indirect, concussive injury to underlying hippocampus and other brain regions, providing a reproducible model for mild traumatic brain injury (mTBI) and its neurochemical, synaptic, and behavioral sequelae. Here, we extend a preliminary gene expression study of the hippocampus-specific events occurring after mCCI and identify 193 transcripts significantly upregulated, and 21 transcripts significantly downregulated, 24 h after mCCI. Fifty-three percent of genes altered by mCCI within 24 h of injury are predicted to be expressed only in the non-neuronal/glial cellular compartment, with only 13% predicted to be expressed only in neurons. The set of upregulated genes following mCCI was interrogated using Ingenuity Pathway Analysis (IPA) augmented with manual curation of the literature (190 transcripts accepted for analysis), revealing a core group of 15 first messengers, mostly inflammatory cytokines, predicted to account for >99% of the transcript upregulation occurring 24 h after mCCI. Convergent analysis of predicted transcription factors (TFs) regulating the mCCI target genes, carried out in IPA relative to the entire Affymet...Continue Reading

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Citations

Nov 12, 2016·Experimental Neurology·Ryan S SprisslerMichael F Hammer
Oct 23, 2016·Journal of Neurochemistry·Danielle E JenkinsStephen J Bunn
Feb 23, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Pavlos MyserlisChristopher D Anderson
Feb 7, 2020·Journal of Neurotrauma·Chaitanya InampudiPeter J Crack
Aug 24, 2019·Frontiers in Cellular Neuroscience·Saef IzzyMichael J Whalen
Jul 18, 2021·Acta neurochirurgica·Sami Abu HamdehNiklas Marklund

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Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.