Acute Temporal Profiles of Serum Levels of UCH-L1 and GFAP and Relationships to Neuronal and Astroglial Pathology following Traumatic Brain Injury in Rats

Journal of Neurotrauma
Xian-Jian HuangBruce G Lyeth

Abstract

A number of potential traumatic brain injury (TBI) biomarkers have been proposed and evaluated in the laboratory and clinic. This study investigated the temporal profile of circulating biomarkers of astrocytic and neuronal injury over the first 24 h and relevant histopathological changes after experimental moderate TBI. Twenty male rats were randomly assigned to either moderate parasagittal fluid percussion or sham injury. Blood serum samples were collected 2 d prior to TBI (baseline) and at 3, 6, and 24 h after TBI. A single cerebrospinal fluid (CSF) sample was collected from the cisterna magna 24 h after TBI, followed by euthanasia and brain harvesting for histology. Serum and CSF samples were analyzed for neuronal (ubiquitin carboxy-terminal hydrolase L1 [UCH-L1]) and astroglial (glial fibrillary acidic protein [GFAP]) protein levels using enzyme-linked immunosorbent assay. Brain histology included GFAP immunostaining and Fluoro-Jade histofluorescence. Serum and CSF levels of GFAP were near zero in sham animals. Serum GFAP levels were significantly elevated at 3 and 6 h post-TBI, compared with baseline and time-matched sham values, while UCH-L1 was significantly elevated only at 3 h post-TBI. Both CSF GFAP and UCH-L1 at 24 h...Continue Reading

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Citations

Nov 7, 2015·Journal of Neurotrauma·Andrea MountneyDeborah Ann Shear
Mar 15, 2016·Frontiers in Integrative Neuroscience·Venkata S S S SajjaPamela J VandeVord
Oct 13, 2015·Critical Care : the Official Journal of the Critical Care Forum·Jin LeiJiyao Jiang
Jan 31, 2018·Journal of Neuroscience Research·Janet D PierceJanna L Harris
Sep 27, 2018·Experimental Biology and Medicine·Abigail L WalkerRuth A Roberts
Oct 6, 2017·Brain Injury : [BI]·Denes V AgostonElaine R Peskind
Aug 14, 2020·The Journal of the American Osteopathic Association·James A D BerryDan Miulli
Nov 1, 2017·Brain Injury : [BI]·Shima ShahjoueiKevin K W Wang
Oct 30, 2020·Journal of Neurotrauma·Steven T DeKoskyRonald L Hayes
Jul 7, 2019·The Journal of Surgical Research·Mackenzie C MorrisMichael D Goodman

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
blood draw
ELISA
enzyme-linked immunosorbent assay

Software Mentioned

SigmaPlot
SPSS
GrapPad Prism

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