Acute toxicity profile of cadmium revealed by proteomics in brain tissue of Paralichthys olivaceus: potential role of transferrin in cadmium toxicity

Aquatic Toxicology
Jin-Yong ZhuZongwei Cai

Abstract

An analytical approach using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) separated proteins from the brain tissue of the fish Paralichthys olivaceus. Approximately 600 protein spots were detected from the brain sample when applying 600 microg protein to a 2D-PAGE gel in the pH range 3.5-10.0. Compared to a control sample, significant changes of 24 protein spots were observed in the fish tissue exposed to acute toxicity of seawater cadmium (SCAT) at 10 ppm for 24h. Among these spots, nine were down-regulated, nine were up-regulated, two showed high expression, and four showed low expression. The collected spots were identified by peptide mass fingerprinting (PMF) and database search, and they were further classified by LOC tree, a hierarchical system of support vector machines which predict their sub-cellular localization. The amount of transferrin expression in brain cells decreased linearly with the increase of SCAT concentration in seawater. Among the 24 proteins identified on a 2D-PAGE gel, 9 demonstrated a synchronous response to acute cadmium, suggesting that they might represent a biomarker profile. Based on their variable levels and trends on the 2D-PAGE gel this protein (likely to be transferrin) sugges...Continue Reading

Citations

Oct 7, 2009·Ecotoxicology·Caroline Geeraerts, Claude Belpaire
Nov 15, 2006·Photosynthesis Research·Sylvie GilletPierre Le Maréchal
Jul 7, 2012·Environmental Science and Pollution Research International·Xue-Ping LingHe-Qing Huang
Jun 4, 2013·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Peng ZhangTaiwu Li
Oct 1, 2014·Acta Biochimica Et Biophysica Sinica·Xinyi HeXingmeng Lu
Jan 16, 2016·Environmental Toxicology and Chemistry·Paulina A BahamondeChristopher J Martyniuk
Jul 11, 2012·Marine Environmental Research·Natacha JeanCédric Garnier
Apr 7, 2012·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Marleen EyckmansGudrun De Boeck
Dec 29, 2011·General and Comparative Endocrinology·Christopher J MartyniukVance L Trudeau
Oct 22, 2011·Blood Cells, Molecules & Diseases·Jin-Yu HuYing-Wei Wang
Sep 10, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Qing-Yu HuangHe-Qing Huang
Mar 1, 2009·Environmental Toxicology and Pharmacology·Xue-Ping LingHe-Qing Huang
Dec 7, 2007·Animal Reproduction Science·Saeed Keyvanshokooh, Behrouz Vaziri
Nov 13, 2010·Environmental Toxicology and Chemistry·Brian C SanchezMaría S Sepúlveda
Dec 3, 2009·Proteomics·Marco F L LemosAna C Esteves
Dec 3, 2009·Proteomics·Ignasi FornéJoan Cerdà
Apr 14, 2012·Journal of Proteomics·Pedro M RodriguesFlemming Jessen
Mar 21, 2015·PloS One·Yoshiharu YamanobeToshihiro Takizawa
Jan 26, 2017·Photochemistry and Photobiology·Xi PengYang Gao
Feb 15, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Gisela LannigInna M Sokolova
Sep 4, 2012·Metallomics : Integrated Biometal Science·Jin Yong Zhu, King Ming Chan
Aug 18, 2020·Biological Trace Element Research·Xi SunHaifeng Shi
Feb 25, 2014·Molecular Biology Reports·Ji LiuXiaoming Deng
Dec 4, 2019·The Science of the Total Environment·Diana BarrosFernanda Cássio
Mar 10, 2009·Journal of Proteome Research·Christopher J MartyniukNancy D Denslow
Nov 13, 2010·Journal of Proteome Research·Jennifer DortsFrédéric Silvestre
Feb 18, 2014·Journal of Proteome Research·John M PrinsYinsheng Wang
Jan 22, 2009·Analytica Chimica Acta·M Estela del Castillo BustoAlfredo Sanz-Medel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.