Acute versus chronic phase mechanisms in a rat model of CRPS

Journal of Neuroinflammation
Tzuping WeiJohn David Clark

Abstract

Tibia fracture followed by cast immobilization in rats evokes nociceptive, vascular, epidermal, and bone changes resembling complex regional pain syndrome (CRPS). In most cases, CRPS has three stages. Over time, this acute picture, allodynia, warmth, and edema observed at 4 weeks, gives way to a cold, dystrophic but still painful limb. In the acute phase (at 4 weeks post fracture), cutaneous immunological and NK1-receptor signaling mechanisms underlying CRPS have been discovered; however, the mechanisms responsible for the chronic phase are still unknown. The purpose of this study is to understand the mechanisms responsible for the chronic phases of CRPS (at 16 weeks post fracture) at both the peripheral and central levels. We used rat tibial fracture/cast immobilization model of CRPS to study molecular, vascular, and nociceptive changes at 4 and 16 weeks post fracture. Immunoassays and Western blotting were carried out to monitor changes in inflammatory response and NK1-receptor signaling in the skin and spinal cord. Skin temperature and thickness were measured to elucidate vascular changes, whereas von Frey testing and unweighting were carried out to study nociceptive changes. All data were analyzed by one-way analysis of var...Continue Reading

References

May 1, 1989·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·R M AtkinsJ A Kanis
Oct 1, 1989·British Journal of Rheumatology·R M Atkins, J A Kanis
Mar 1, 1994·British Journal of Rheumatology·D R Bickerstaff, J A Kanis
May 1, 1994·The Journal of Clinical Investigation·R B KimbleR Pacifici
May 1, 1993·The Journal of Bone and Joint Surgery. British Volume·P P SarangiR M Atkins
Feb 2, 1996·Journal of Medicinal Chemistry·P A HipskindB D Gitter
Dec 4, 2003·Expert Opinion on Pharmacotherapy·Lisa Patel, Celeste Lindley
May 26, 2004·Journal of Pain and Symptom Management·Frank J P M HuygenPaul L A van Daele
Feb 21, 2006·Mediators of Inflammation·Renate J M MunnikesFrank J P M Huygen
Jul 6, 2007·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·R Norman HardenPeter R Wilson
Apr 22, 2008·Neuroscience Letters·Frank Birklein, Martin Schmelz
Mar 20, 2010·Journal of the International Neuropsychological Society : JINS·David J LibonAtul Kalanuria
Aug 28, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Mark S Cooper, Vincent P Clark
Nov 29, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·C LinnmanD Borsook
Mar 5, 2013·The Journal of Pain : Official Journal of the American Pain Society·Alison PepperJ David Clark
Jan 28, 2014·The Journal of Pain : Official Journal of the American Pain Society·Frank BirkleinWade S Kingery
Dec 5, 2014·Neurology·Frank BirkleinTanja Schlereth

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Citations

Feb 21, 2018·Anesthesiology·Ru-Rong JiWilliam Maixner
Feb 23, 2020·Muscle & Nerve·Satoshi OgaMinoru Okita
Aug 15, 2017·Expert Review of Proteomics·Simone KönigFrank Birklein
Nov 19, 2018·Journal of Anesthesia·Megumi MatsudaRu-Rong Ji
Jul 28, 2019·British Journal of Pharmacology·Thomas M Tzschentke
Jul 31, 2020·International Journal of Molecular Sciences·Ramona D'AmicoRosanna Di Paola
Dec 16, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Karen Binkley, Rita Katznelson
May 2, 2018·The Journal of Pain : Official Journal of the American Pain Society·Tian-Zhi GuoJohn David Clark
May 14, 2021·Pain Reports·Michael J LacagninaPeter M Grace

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Methods Mentioned

BETA
confocal microscopy
Protein Assay

Software Mentioned

LSM Image Browser
Prism
LSM Data Server
ImageQuant
Zeiss
GraphPad

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